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Inducible Genetic Code Expansion in Eukaryotes

Genetic code expansion (GCE) is a versatile tool to site‐specifically incorporate a noncanonical amino acid (ncAA) into a protein, for example, to perform fluorescent labeling inside living cells. To this end, an orthogonal aminoacyl‐tRNA‐synthetase/tRNA (RS/tRNA) pair is used to insert the ncAA in...

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Detalles Bibliográficos
Autores principales: Koehler, Christine, Estrada Girona, Gemma, Reinkemeier, Christopher D., Lemke, Edward A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7754456/
https://www.ncbi.nlm.nih.gov/pubmed/32598534
http://dx.doi.org/10.1002/cbic.202000338