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Poly(alizarin red S) modified glassy carbon electrode for square wave adsorptive stripping voltammetric determination of metronidazole in tablet formulation
Potentiodynamically fabricated poly(alizarin red s) modified GCE was characterized using CV and EIS techniques. In contrast to the cyclic voltammetric response of the unmodified GCE for metronidazole, an irreversible reduction peak with three-folds of current enhancement and reduced overpotential at...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755201/ https://www.ncbi.nlm.nih.gov/pubmed/33351825 http://dx.doi.org/10.1371/journal.pone.0244115 |
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author | Dawit, Mulugeta Turbale, Mahilet Moges, Amsalu Amare, Meareg |
author_facet | Dawit, Mulugeta Turbale, Mahilet Moges, Amsalu Amare, Meareg |
author_sort | Dawit, Mulugeta |
collection | PubMed |
description | Potentiodynamically fabricated poly(alizarin red s) modified GCE was characterized using CV and EIS techniques. In contrast to the cyclic voltammetric response of the unmodified GCE for metronidazole, an irreversible reduction peak with three-folds of current enhancement and reduced overpotential at the poly(alizarin red s) modified GCE showed the catalytic effect of the modifier towards reduction of metronidazole. While observed peak potential shift with increasing pH (4.0–10.0) indicated the involvement of protons during the reduction of metronidazole, peak potential shift with scan rate (20–300 mV s(-1)) confirmed the irreversibility of the reduction reaction of metronidazole at the modified GCE. A better correlation for the dependence of peak current on scan rate (r(2) = 0.9883) than on square root of scan rate (r(2) = 0.9740) supplemented by slope value of 0.38 for plot of log(current) versus log(scan rate) indicated the reduction reaction of metronidazole at the surface of the modified electrode was predominantly adsorption controlled. Under the optimized method and solution parameters, reductive current response of tablet sample showed linear dependence on spiked standard concentration in a wide range (0–125 μM) with excellent determination coefficient r(2), LoD and LoQ of 0.9991, 0.38, and 1.25 μM, respectively. Spike recovery of 97.9% and interference recovery of 96.2–97.5% in the presence of 21.28 and 31.92 μM of uric acid and ascorbic acid validated the applicability of the present method for determination of metronidazole in tablet formulation. The metronidazole content of the tested tablet formulation using standard addition method was found to be 97.6% of what is claimed by the tablet manufacturer making the developed method an excellent potential candidate for its applicability to determine metronidazole in real samples with complex matrix. |
format | Online Article Text |
id | pubmed-7755201 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-77552012021-01-05 Poly(alizarin red S) modified glassy carbon electrode for square wave adsorptive stripping voltammetric determination of metronidazole in tablet formulation Dawit, Mulugeta Turbale, Mahilet Moges, Amsalu Amare, Meareg PLoS One Research Article Potentiodynamically fabricated poly(alizarin red s) modified GCE was characterized using CV and EIS techniques. In contrast to the cyclic voltammetric response of the unmodified GCE for metronidazole, an irreversible reduction peak with three-folds of current enhancement and reduced overpotential at the poly(alizarin red s) modified GCE showed the catalytic effect of the modifier towards reduction of metronidazole. While observed peak potential shift with increasing pH (4.0–10.0) indicated the involvement of protons during the reduction of metronidazole, peak potential shift with scan rate (20–300 mV s(-1)) confirmed the irreversibility of the reduction reaction of metronidazole at the modified GCE. A better correlation for the dependence of peak current on scan rate (r(2) = 0.9883) than on square root of scan rate (r(2) = 0.9740) supplemented by slope value of 0.38 for plot of log(current) versus log(scan rate) indicated the reduction reaction of metronidazole at the surface of the modified electrode was predominantly adsorption controlled. Under the optimized method and solution parameters, reductive current response of tablet sample showed linear dependence on spiked standard concentration in a wide range (0–125 μM) with excellent determination coefficient r(2), LoD and LoQ of 0.9991, 0.38, and 1.25 μM, respectively. Spike recovery of 97.9% and interference recovery of 96.2–97.5% in the presence of 21.28 and 31.92 μM of uric acid and ascorbic acid validated the applicability of the present method for determination of metronidazole in tablet formulation. The metronidazole content of the tested tablet formulation using standard addition method was found to be 97.6% of what is claimed by the tablet manufacturer making the developed method an excellent potential candidate for its applicability to determine metronidazole in real samples with complex matrix. Public Library of Science 2020-12-22 /pmc/articles/PMC7755201/ /pubmed/33351825 http://dx.doi.org/10.1371/journal.pone.0244115 Text en © 2020 Dawit et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Dawit, Mulugeta Turbale, Mahilet Moges, Amsalu Amare, Meareg Poly(alizarin red S) modified glassy carbon electrode for square wave adsorptive stripping voltammetric determination of metronidazole in tablet formulation |
title | Poly(alizarin red S) modified glassy carbon electrode for square wave adsorptive stripping voltammetric determination of metronidazole in tablet formulation |
title_full | Poly(alizarin red S) modified glassy carbon electrode for square wave adsorptive stripping voltammetric determination of metronidazole in tablet formulation |
title_fullStr | Poly(alizarin red S) modified glassy carbon electrode for square wave adsorptive stripping voltammetric determination of metronidazole in tablet formulation |
title_full_unstemmed | Poly(alizarin red S) modified glassy carbon electrode for square wave adsorptive stripping voltammetric determination of metronidazole in tablet formulation |
title_short | Poly(alizarin red S) modified glassy carbon electrode for square wave adsorptive stripping voltammetric determination of metronidazole in tablet formulation |
title_sort | poly(alizarin red s) modified glassy carbon electrode for square wave adsorptive stripping voltammetric determination of metronidazole in tablet formulation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755201/ https://www.ncbi.nlm.nih.gov/pubmed/33351825 http://dx.doi.org/10.1371/journal.pone.0244115 |
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