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Analysis of the vaginal microbiome of giant pandas using metagenomics sequencing

In this study, a total of 14 vaginal samples (GPV1‐14) from giant pandas were analyzed. These vaginal samples were divided into two groups as per the region and age of giant pandas. All the vaginal samples were analyzed using metagenomic sequencing. As per the outcomes of metagenomic analysis, Prote...

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Detalles Bibliográficos
Autores principales: Zhang, Lan, Li, Caiwu, Zhai, Yaru, Feng, Lan, Bai, Keke, Zhang, Zhizhong, Huang, Yan, Li, Ti, Li, Desheng, Li, Hao, Cui, Pengfei, Chen, Danyu, Wang, Hongning, Yang, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755806/
https://www.ncbi.nlm.nih.gov/pubmed/33205903
http://dx.doi.org/10.1002/mbo3.1131
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author Zhang, Lan
Li, Caiwu
Zhai, Yaru
Feng, Lan
Bai, Keke
Zhang, Zhizhong
Huang, Yan
Li, Ti
Li, Desheng
Li, Hao
Cui, Pengfei
Chen, Danyu
Wang, Hongning
Yang, Xin
author_facet Zhang, Lan
Li, Caiwu
Zhai, Yaru
Feng, Lan
Bai, Keke
Zhang, Zhizhong
Huang, Yan
Li, Ti
Li, Desheng
Li, Hao
Cui, Pengfei
Chen, Danyu
Wang, Hongning
Yang, Xin
author_sort Zhang, Lan
collection PubMed
description In this study, a total of 14 vaginal samples (GPV1‐14) from giant pandas were analyzed. These vaginal samples were divided into two groups as per the region and age of giant pandas. All the vaginal samples were analyzed using metagenomic sequencing. As per the outcomes of metagenomic analysis, Proteobacteria (39.04%), Firmicutes (5.27%), Actinobacteria (2.94%), and Basidiomycota (2.77%) were found to be the dominant phyla in the microbiome of the vaginal samples. At the genus level, Pseudomonas (21.90%) was found to be the most dominant genus, followed by Streptococcus (3.47%), Psychrobacter (1.89%), and Proteus (1.38%). Metastats analysis of the microbial species in the vaginal samples of giant pandas from Wolong Nature Reserve, Dujiangyan and Ningbo Youngor Zoo, and Ya'an Bifengxia Nature Reserve was found to be significantly different (p < 0.05). Age groups, that is, AGE1 (5‐10 years old) and AGE2 (11‐16 years old), also demonstrated significantly different inter‐group microbial species (p < 0.05). For the first time, Chlamydia and Neisseria gonorrhoeae were detected in giant pandas’ reproductive tract. GPV3 vaginal sample (2.63%) showed highest Chlamydia content followed by GPV14 (0.91%), and GPV7 (0.62%). GPV5 vaginal sample (7.17%) showed the highest Neisseria gonorrhoeae content, followed by GPV14 (7.02%), and GPV8 (6.50%). Furthermore, we employed eggNOG, CAZy, KEGG, and NCBI databases to investigate the functional significance of giant panda's vaginal microbial community. The outcomes indicated that giant panda's vaginal microbes were involved in biological processes. The data from this study will help in improving the reproductive health of giant pandas.
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spelling pubmed-77558062020-12-23 Analysis of the vaginal microbiome of giant pandas using metagenomics sequencing Zhang, Lan Li, Caiwu Zhai, Yaru Feng, Lan Bai, Keke Zhang, Zhizhong Huang, Yan Li, Ti Li, Desheng Li, Hao Cui, Pengfei Chen, Danyu Wang, Hongning Yang, Xin Microbiologyopen Original Articles In this study, a total of 14 vaginal samples (GPV1‐14) from giant pandas were analyzed. These vaginal samples were divided into two groups as per the region and age of giant pandas. All the vaginal samples were analyzed using metagenomic sequencing. As per the outcomes of metagenomic analysis, Proteobacteria (39.04%), Firmicutes (5.27%), Actinobacteria (2.94%), and Basidiomycota (2.77%) were found to be the dominant phyla in the microbiome of the vaginal samples. At the genus level, Pseudomonas (21.90%) was found to be the most dominant genus, followed by Streptococcus (3.47%), Psychrobacter (1.89%), and Proteus (1.38%). Metastats analysis of the microbial species in the vaginal samples of giant pandas from Wolong Nature Reserve, Dujiangyan and Ningbo Youngor Zoo, and Ya'an Bifengxia Nature Reserve was found to be significantly different (p < 0.05). Age groups, that is, AGE1 (5‐10 years old) and AGE2 (11‐16 years old), also demonstrated significantly different inter‐group microbial species (p < 0.05). For the first time, Chlamydia and Neisseria gonorrhoeae were detected in giant pandas’ reproductive tract. GPV3 vaginal sample (2.63%) showed highest Chlamydia content followed by GPV14 (0.91%), and GPV7 (0.62%). GPV5 vaginal sample (7.17%) showed the highest Neisseria gonorrhoeae content, followed by GPV14 (7.02%), and GPV8 (6.50%). Furthermore, we employed eggNOG, CAZy, KEGG, and NCBI databases to investigate the functional significance of giant panda's vaginal microbial community. The outcomes indicated that giant panda's vaginal microbes were involved in biological processes. The data from this study will help in improving the reproductive health of giant pandas. John Wiley and Sons Inc. 2020-11-18 /pmc/articles/PMC7755806/ /pubmed/33205903 http://dx.doi.org/10.1002/mbo3.1131 Text en © 2020 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Zhang, Lan
Li, Caiwu
Zhai, Yaru
Feng, Lan
Bai, Keke
Zhang, Zhizhong
Huang, Yan
Li, Ti
Li, Desheng
Li, Hao
Cui, Pengfei
Chen, Danyu
Wang, Hongning
Yang, Xin
Analysis of the vaginal microbiome of giant pandas using metagenomics sequencing
title Analysis of the vaginal microbiome of giant pandas using metagenomics sequencing
title_full Analysis of the vaginal microbiome of giant pandas using metagenomics sequencing
title_fullStr Analysis of the vaginal microbiome of giant pandas using metagenomics sequencing
title_full_unstemmed Analysis of the vaginal microbiome of giant pandas using metagenomics sequencing
title_short Analysis of the vaginal microbiome of giant pandas using metagenomics sequencing
title_sort analysis of the vaginal microbiome of giant pandas using metagenomics sequencing
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755806/
https://www.ncbi.nlm.nih.gov/pubmed/33205903
http://dx.doi.org/10.1002/mbo3.1131
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