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Identification of gut microbiota and microbial metabolites regulated by an anti-microbial peptide lipocalin 2 in high fat diet-induced obesity

Lipocalin 2 (Lcn2), as an anti-microbial peptide is expressed in intestine, and the upregulation of intestinal Lcn2 has been linked to inflammatory bowel disease. However, the role of Lcn2 in shaping gut microbiota during diet-induced obesity (DIO) remains unknown. We found that short-term high fat...

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Autores principales: Qiu, Xiaoxue, Macchietto, Marissa, Liu, Xiaotong, Lu, You, Ma, Yiwei, Guo, Hong, Saqui-Salces, Milena, Bernlohr, David A, Chen, Chi, Shen, Steven, Chen, Xiaoli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755824/
https://www.ncbi.nlm.nih.gov/pubmed/33214705
http://dx.doi.org/10.1038/s41366-020-00712-2
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author Qiu, Xiaoxue
Macchietto, Marissa
Liu, Xiaotong
Lu, You
Ma, Yiwei
Guo, Hong
Saqui-Salces, Milena
Bernlohr, David A
Chen, Chi
Shen, Steven
Chen, Xiaoli
author_facet Qiu, Xiaoxue
Macchietto, Marissa
Liu, Xiaotong
Lu, You
Ma, Yiwei
Guo, Hong
Saqui-Salces, Milena
Bernlohr, David A
Chen, Chi
Shen, Steven
Chen, Xiaoli
author_sort Qiu, Xiaoxue
collection PubMed
description Lipocalin 2 (Lcn2), as an anti-microbial peptide is expressed in intestine, and the upregulation of intestinal Lcn2 has been linked to inflammatory bowel disease. However, the role of Lcn2 in shaping gut microbiota during diet-induced obesity (DIO) remains unknown. We found that short-term high fat diet (HFD) feeding strongly stimulates intestinal Lcn2 expression and secretion into the gut lumen. As the HFD feeding prolongs, fecal Lcn2 levels turn to decrease. Lcn2 deficiency accelerates the development of HFD-induced intestinal inflammation and microbiota dysbiosis. Moreover, Lcn2 deficiency leads to the remodeling of microbiota-derived metabolome, including decreased production of short-chain fatty acids (SCFAs) and SCFA-producing microbes. Most importantly, we have identified Lcn2-targeted bacteria and microbiota-derived metabolites that potentially play roles in DIO and metabolic dysregulation. Correlation analyses suggest that Lcn2-targeted Dubosiella and Angelakisella have a novel role in regulating SCFAs production and obesity. Our results provide a novel mechanism involving Lcn2 as an anti-microbial host factor in the control of gut microbiota symbiosis during DIO.
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spelling pubmed-77558242021-05-19 Identification of gut microbiota and microbial metabolites regulated by an anti-microbial peptide lipocalin 2 in high fat diet-induced obesity Qiu, Xiaoxue Macchietto, Marissa Liu, Xiaotong Lu, You Ma, Yiwei Guo, Hong Saqui-Salces, Milena Bernlohr, David A Chen, Chi Shen, Steven Chen, Xiaoli Int J Obes (Lond) Article Lipocalin 2 (Lcn2), as an anti-microbial peptide is expressed in intestine, and the upregulation of intestinal Lcn2 has been linked to inflammatory bowel disease. However, the role of Lcn2 in shaping gut microbiota during diet-induced obesity (DIO) remains unknown. We found that short-term high fat diet (HFD) feeding strongly stimulates intestinal Lcn2 expression and secretion into the gut lumen. As the HFD feeding prolongs, fecal Lcn2 levels turn to decrease. Lcn2 deficiency accelerates the development of HFD-induced intestinal inflammation and microbiota dysbiosis. Moreover, Lcn2 deficiency leads to the remodeling of microbiota-derived metabolome, including decreased production of short-chain fatty acids (SCFAs) and SCFA-producing microbes. Most importantly, we have identified Lcn2-targeted bacteria and microbiota-derived metabolites that potentially play roles in DIO and metabolic dysregulation. Correlation analyses suggest that Lcn2-targeted Dubosiella and Angelakisella have a novel role in regulating SCFAs production and obesity. Our results provide a novel mechanism involving Lcn2 as an anti-microbial host factor in the control of gut microbiota symbiosis during DIO. 2020-11-19 2021-01 /pmc/articles/PMC7755824/ /pubmed/33214705 http://dx.doi.org/10.1038/s41366-020-00712-2 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Qiu, Xiaoxue
Macchietto, Marissa
Liu, Xiaotong
Lu, You
Ma, Yiwei
Guo, Hong
Saqui-Salces, Milena
Bernlohr, David A
Chen, Chi
Shen, Steven
Chen, Xiaoli
Identification of gut microbiota and microbial metabolites regulated by an anti-microbial peptide lipocalin 2 in high fat diet-induced obesity
title Identification of gut microbiota and microbial metabolites regulated by an anti-microbial peptide lipocalin 2 in high fat diet-induced obesity
title_full Identification of gut microbiota and microbial metabolites regulated by an anti-microbial peptide lipocalin 2 in high fat diet-induced obesity
title_fullStr Identification of gut microbiota and microbial metabolites regulated by an anti-microbial peptide lipocalin 2 in high fat diet-induced obesity
title_full_unstemmed Identification of gut microbiota and microbial metabolites regulated by an anti-microbial peptide lipocalin 2 in high fat diet-induced obesity
title_short Identification of gut microbiota and microbial metabolites regulated by an anti-microbial peptide lipocalin 2 in high fat diet-induced obesity
title_sort identification of gut microbiota and microbial metabolites regulated by an anti-microbial peptide lipocalin 2 in high fat diet-induced obesity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755824/
https://www.ncbi.nlm.nih.gov/pubmed/33214705
http://dx.doi.org/10.1038/s41366-020-00712-2
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