Cargando…
Monoacylglycerol Lipase Knockdown Inhibits Cell Proliferation and Metastasis in Lung Adenocarcinoma
Abnormal metabolism is one of the hallmarks of cancer cells. Monoacylglycerol lipase (MGLL), a key enzyme in lipid metabolism, has emerged as an important regulator of tumor progression. In this study, we aimed to characterize the role of MGLL in the development of lung adenocarcinoma (LUAD). To thi...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756122/ https://www.ncbi.nlm.nih.gov/pubmed/33363004 http://dx.doi.org/10.3389/fonc.2020.559568 |
_version_ | 1783626472673509376 |
---|---|
author | Zhang, Hao Guo, Wei Zhang, Fan Li, Renda Zhou, Yang Shao, Fei Feng, Xiaoli Tan, Fengwei Wang, Jie Gao, Shugeng Gao, Yibo He, Jie |
author_facet | Zhang, Hao Guo, Wei Zhang, Fan Li, Renda Zhou, Yang Shao, Fei Feng, Xiaoli Tan, Fengwei Wang, Jie Gao, Shugeng Gao, Yibo He, Jie |
author_sort | Zhang, Hao |
collection | PubMed |
description | Abnormal metabolism is one of the hallmarks of cancer cells. Monoacylglycerol lipase (MGLL), a key enzyme in lipid metabolism, has emerged as an important regulator of tumor progression. In this study, we aimed to characterize the role of MGLL in the development of lung adenocarcinoma (LUAD). To this end, we used tissue microarrays to evaluate the expression of MGLL in LUAD tissue and assessed whether the levels of this protein are correlated with clinicopathological characteristics of LUAD. We found that the expression of MGLL is higher in LUAD samples than that in adjacent non-tumor tissues. In addition, elevated MGLL expression was found to be associated with advanced tumor progression and poor prognosis in LUAD patients. Functional studies further demonstrated that stable short hairpin RNA (shRNA)-mediated knockdown of MGLL inhibits tumor proliferation and metastasis, both in vitro and in vivo, and mechanistically, our data indicate that MGLL regulates Cyclin D1 and Cyclin B1 in LUAD cells. Moreover, we found that knockdown of MGLL suppresses the expression of matrix metalloproteinase 14 (MMP14) in A549 and H322 cells, and in clinical samples, expression of MMP14 is significantly correlated with MGLL expression. Taken together, our results indicate that MGLL plays an oncogenic role in LUAD progression and metastasis and may serve as a potential biomarker for disease prognosis and as a target for the development of personalized therapies. |
format | Online Article Text |
id | pubmed-7756122 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77561222020-12-24 Monoacylglycerol Lipase Knockdown Inhibits Cell Proliferation and Metastasis in Lung Adenocarcinoma Zhang, Hao Guo, Wei Zhang, Fan Li, Renda Zhou, Yang Shao, Fei Feng, Xiaoli Tan, Fengwei Wang, Jie Gao, Shugeng Gao, Yibo He, Jie Front Oncol Oncology Abnormal metabolism is one of the hallmarks of cancer cells. Monoacylglycerol lipase (MGLL), a key enzyme in lipid metabolism, has emerged as an important regulator of tumor progression. In this study, we aimed to characterize the role of MGLL in the development of lung adenocarcinoma (LUAD). To this end, we used tissue microarrays to evaluate the expression of MGLL in LUAD tissue and assessed whether the levels of this protein are correlated with clinicopathological characteristics of LUAD. We found that the expression of MGLL is higher in LUAD samples than that in adjacent non-tumor tissues. In addition, elevated MGLL expression was found to be associated with advanced tumor progression and poor prognosis in LUAD patients. Functional studies further demonstrated that stable short hairpin RNA (shRNA)-mediated knockdown of MGLL inhibits tumor proliferation and metastasis, both in vitro and in vivo, and mechanistically, our data indicate that MGLL regulates Cyclin D1 and Cyclin B1 in LUAD cells. Moreover, we found that knockdown of MGLL suppresses the expression of matrix metalloproteinase 14 (MMP14) in A549 and H322 cells, and in clinical samples, expression of MMP14 is significantly correlated with MGLL expression. Taken together, our results indicate that MGLL plays an oncogenic role in LUAD progression and metastasis and may serve as a potential biomarker for disease prognosis and as a target for the development of personalized therapies. Frontiers Media S.A. 2020-12-09 /pmc/articles/PMC7756122/ /pubmed/33363004 http://dx.doi.org/10.3389/fonc.2020.559568 Text en Copyright © 2020 Zhang, Guo, Zhang, Li, Zhou, Shao, Feng, Tan, Wang, Gao, Gao and He http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Zhang, Hao Guo, Wei Zhang, Fan Li, Renda Zhou, Yang Shao, Fei Feng, Xiaoli Tan, Fengwei Wang, Jie Gao, Shugeng Gao, Yibo He, Jie Monoacylglycerol Lipase Knockdown Inhibits Cell Proliferation and Metastasis in Lung Adenocarcinoma |
title | Monoacylglycerol Lipase Knockdown Inhibits Cell Proliferation and Metastasis in Lung Adenocarcinoma |
title_full | Monoacylglycerol Lipase Knockdown Inhibits Cell Proliferation and Metastasis in Lung Adenocarcinoma |
title_fullStr | Monoacylglycerol Lipase Knockdown Inhibits Cell Proliferation and Metastasis in Lung Adenocarcinoma |
title_full_unstemmed | Monoacylglycerol Lipase Knockdown Inhibits Cell Proliferation and Metastasis in Lung Adenocarcinoma |
title_short | Monoacylglycerol Lipase Knockdown Inhibits Cell Proliferation and Metastasis in Lung Adenocarcinoma |
title_sort | monoacylglycerol lipase knockdown inhibits cell proliferation and metastasis in lung adenocarcinoma |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756122/ https://www.ncbi.nlm.nih.gov/pubmed/33363004 http://dx.doi.org/10.3389/fonc.2020.559568 |
work_keys_str_mv | AT zhanghao monoacylglycerollipaseknockdowninhibitscellproliferationandmetastasisinlungadenocarcinoma AT guowei monoacylglycerollipaseknockdowninhibitscellproliferationandmetastasisinlungadenocarcinoma AT zhangfan monoacylglycerollipaseknockdowninhibitscellproliferationandmetastasisinlungadenocarcinoma AT lirenda monoacylglycerollipaseknockdowninhibitscellproliferationandmetastasisinlungadenocarcinoma AT zhouyang monoacylglycerollipaseknockdowninhibitscellproliferationandmetastasisinlungadenocarcinoma AT shaofei monoacylglycerollipaseknockdowninhibitscellproliferationandmetastasisinlungadenocarcinoma AT fengxiaoli monoacylglycerollipaseknockdowninhibitscellproliferationandmetastasisinlungadenocarcinoma AT tanfengwei monoacylglycerollipaseknockdowninhibitscellproliferationandmetastasisinlungadenocarcinoma AT wangjie monoacylglycerollipaseknockdowninhibitscellproliferationandmetastasisinlungadenocarcinoma AT gaoshugeng monoacylglycerollipaseknockdowninhibitscellproliferationandmetastasisinlungadenocarcinoma AT gaoyibo monoacylglycerollipaseknockdowninhibitscellproliferationandmetastasisinlungadenocarcinoma AT hejie monoacylglycerollipaseknockdowninhibitscellproliferationandmetastasisinlungadenocarcinoma |