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Cell-free DNA (cfDNA) and Exosome Profiling from a Year-Long Human Spaceflight Reveals Circulating Biomarkers
Liquid biopsies based on cell-free DNA (cfDNA) or exosomes provide a noninvasive approach to monitor human health and disease but have not been utilized for astronauts. Here, we profile cfDNA characteristics, including fragment size, cellular deconvolution, and nucleosome positioning, in an astronau...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756145/ https://www.ncbi.nlm.nih.gov/pubmed/33376973 http://dx.doi.org/10.1016/j.isci.2020.101844 |
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author | Bezdan, Daniela Grigorev, Kirill Meydan, Cem Pelissier Vatter, Fanny A. Cioffi, Michele Rao, Varsha MacKay, Matthew Nakahira, Kiichi Burnham, Philip Afshinnekoo, Ebrahim Westover, Craig Butler, Daniel Mozsary, Chris Donahoe, Timothy Foox, Jonathan Mishra, Tejaswini Lucotti, Serena Rana, Brinda K. Melnick, Ari M. Zhang, Haiying Matei, Irina Kelsen, David Yu, Kenneth Lyden, David C. Taylor, Lynn Bailey, Susan M. Snyder, Michael P. Garrett-Bakelman, Francine E. Ossowski, Stephan De Vlaminck, Iwijn Mason, Christopher E. |
author_facet | Bezdan, Daniela Grigorev, Kirill Meydan, Cem Pelissier Vatter, Fanny A. Cioffi, Michele Rao, Varsha MacKay, Matthew Nakahira, Kiichi Burnham, Philip Afshinnekoo, Ebrahim Westover, Craig Butler, Daniel Mozsary, Chris Donahoe, Timothy Foox, Jonathan Mishra, Tejaswini Lucotti, Serena Rana, Brinda K. Melnick, Ari M. Zhang, Haiying Matei, Irina Kelsen, David Yu, Kenneth Lyden, David C. Taylor, Lynn Bailey, Susan M. Snyder, Michael P. Garrett-Bakelman, Francine E. Ossowski, Stephan De Vlaminck, Iwijn Mason, Christopher E. |
author_sort | Bezdan, Daniela |
collection | PubMed |
description | Liquid biopsies based on cell-free DNA (cfDNA) or exosomes provide a noninvasive approach to monitor human health and disease but have not been utilized for astronauts. Here, we profile cfDNA characteristics, including fragment size, cellular deconvolution, and nucleosome positioning, in an astronaut during a year-long mission on the International Space Station, compared to his identical twin on Earth and healthy donors. We observed a significant increase in the proportion of cell-free mitochondrial DNA (cf-mtDNA) inflight, and analysis of post-flight exosomes in plasma revealed a 30-fold increase in circulating exosomes and patient-specific protein cargo (including brain-derived peptides) after the year-long mission. This longitudinal analysis of astronaut cfDNA during spaceflight and the exosome profiles highlights their utility for astronaut health monitoring, as well as cf-mtDNA levels as a potential biomarker for physiological stress or immune system responses related to microgravity, radiation exposure, and the other unique environmental conditions of spaceflight. |
format | Online Article Text |
id | pubmed-7756145 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-77561452020-12-28 Cell-free DNA (cfDNA) and Exosome Profiling from a Year-Long Human Spaceflight Reveals Circulating Biomarkers Bezdan, Daniela Grigorev, Kirill Meydan, Cem Pelissier Vatter, Fanny A. Cioffi, Michele Rao, Varsha MacKay, Matthew Nakahira, Kiichi Burnham, Philip Afshinnekoo, Ebrahim Westover, Craig Butler, Daniel Mozsary, Chris Donahoe, Timothy Foox, Jonathan Mishra, Tejaswini Lucotti, Serena Rana, Brinda K. Melnick, Ari M. Zhang, Haiying Matei, Irina Kelsen, David Yu, Kenneth Lyden, David C. Taylor, Lynn Bailey, Susan M. Snyder, Michael P. Garrett-Bakelman, Francine E. Ossowski, Stephan De Vlaminck, Iwijn Mason, Christopher E. iScience Article Liquid biopsies based on cell-free DNA (cfDNA) or exosomes provide a noninvasive approach to monitor human health and disease but have not been utilized for astronauts. Here, we profile cfDNA characteristics, including fragment size, cellular deconvolution, and nucleosome positioning, in an astronaut during a year-long mission on the International Space Station, compared to his identical twin on Earth and healthy donors. We observed a significant increase in the proportion of cell-free mitochondrial DNA (cf-mtDNA) inflight, and analysis of post-flight exosomes in plasma revealed a 30-fold increase in circulating exosomes and patient-specific protein cargo (including brain-derived peptides) after the year-long mission. This longitudinal analysis of astronaut cfDNA during spaceflight and the exosome profiles highlights their utility for astronaut health monitoring, as well as cf-mtDNA levels as a potential biomarker for physiological stress or immune system responses related to microgravity, radiation exposure, and the other unique environmental conditions of spaceflight. Elsevier 2020-11-25 /pmc/articles/PMC7756145/ /pubmed/33376973 http://dx.doi.org/10.1016/j.isci.2020.101844 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Bezdan, Daniela Grigorev, Kirill Meydan, Cem Pelissier Vatter, Fanny A. Cioffi, Michele Rao, Varsha MacKay, Matthew Nakahira, Kiichi Burnham, Philip Afshinnekoo, Ebrahim Westover, Craig Butler, Daniel Mozsary, Chris Donahoe, Timothy Foox, Jonathan Mishra, Tejaswini Lucotti, Serena Rana, Brinda K. Melnick, Ari M. Zhang, Haiying Matei, Irina Kelsen, David Yu, Kenneth Lyden, David C. Taylor, Lynn Bailey, Susan M. Snyder, Michael P. Garrett-Bakelman, Francine E. Ossowski, Stephan De Vlaminck, Iwijn Mason, Christopher E. Cell-free DNA (cfDNA) and Exosome Profiling from a Year-Long Human Spaceflight Reveals Circulating Biomarkers |
title | Cell-free DNA (cfDNA) and Exosome Profiling from a Year-Long Human Spaceflight Reveals Circulating Biomarkers |
title_full | Cell-free DNA (cfDNA) and Exosome Profiling from a Year-Long Human Spaceflight Reveals Circulating Biomarkers |
title_fullStr | Cell-free DNA (cfDNA) and Exosome Profiling from a Year-Long Human Spaceflight Reveals Circulating Biomarkers |
title_full_unstemmed | Cell-free DNA (cfDNA) and Exosome Profiling from a Year-Long Human Spaceflight Reveals Circulating Biomarkers |
title_short | Cell-free DNA (cfDNA) and Exosome Profiling from a Year-Long Human Spaceflight Reveals Circulating Biomarkers |
title_sort | cell-free dna (cfdna) and exosome profiling from a year-long human spaceflight reveals circulating biomarkers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756145/ https://www.ncbi.nlm.nih.gov/pubmed/33376973 http://dx.doi.org/10.1016/j.isci.2020.101844 |
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