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Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense

There have been relatively few studies which support a link between Ganoderma boninense, a phytopathogenic fungus that is particularly cytotoxic and pathogenic to plant tissues and roots, and antimicrobial compounds. We previously observed that liquid-liquid extraction (LLE) using chloroformmethanol...

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Autores principales: Abdullah, Syahriel, Oh, Yoon Sin, Kwak, Min-Kyu, Chong, KhimPhin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Microbiological Society of Korea 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756191/
https://www.ncbi.nlm.nih.gov/pubmed/33355891
http://dx.doi.org/10.1007/s12275-021-0551-8
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author Abdullah, Syahriel
Oh, Yoon Sin
Kwak, Min-Kyu
Chong, KhimPhin
author_facet Abdullah, Syahriel
Oh, Yoon Sin
Kwak, Min-Kyu
Chong, KhimPhin
author_sort Abdullah, Syahriel
collection PubMed
description There have been relatively few studies which support a link between Ganoderma boninense, a phytopathogenic fungus that is particularly cytotoxic and pathogenic to plant tissues and roots, and antimicrobial compounds. We previously observed that liquid-liquid extraction (LLE) using chloroformmethanol-water at a ratio (1:1:1) was superior at detecting antibacterial activities and significant quantities of antibacterial compounds. Herein, we demonstrate that antibacterial secondary metabolites are produced from G. boninense mycelia. Antibacterial compounds were monitored in concurrent biochemical and biophysical experiments. The combined methods included high performance thin-layer chromatography (HPTLC), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR) spectroscopy. The antibacterial compounds derived from mycelia with chloroform-methanol extraction through LLE were isolated via a gradient solvent elution system using HPTLC. The antibacterial activity of the isolated compounds was observed to be the most potent against Staphylococcus aureus ATCC 25923 and multidrug-resistant S. aureus NCTC 11939. GC-MS, HPLC, and FTIR analysis confirmed two antibacterial compounds, which were identified as 4,4,14α-trimethylcholestane (m/z = 414.75; lanostane, C(30)H(54)) and ergosta-5,7,22-trien-3β-ol (m/z = 396.65; ergosterol, C(28)H(44)O). With the aid of spectroscopic evaluations, ganoboninketal (m/z = 498.66, C(30)H(42)O(6)), which belongs to the 3,4-seco-27-norlanostane triterpene family, was additionally characterized by 2D-NMR analysis. Despite the lack of antibacterial potential exhibited by lanostane; both ergosterol and ganoboninketal displayed significant antibacterial activities against bacterial pathogens. Results provide evidence for the existence of bioactive compounds in the mycelia of the relatively unexplored phytopathogenic G. boninense, together with a robust method for estimating the corresponding potent antibacterial secondary metabolites. ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available for this article at 10.1007/s12275-021-0551-8 and is accessible for authorized users.
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spelling pubmed-77561912020-12-23 Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense Abdullah, Syahriel Oh, Yoon Sin Kwak, Min-Kyu Chong, KhimPhin J Microbiol Microbial Physiology and Biochemistry There have been relatively few studies which support a link between Ganoderma boninense, a phytopathogenic fungus that is particularly cytotoxic and pathogenic to plant tissues and roots, and antimicrobial compounds. We previously observed that liquid-liquid extraction (LLE) using chloroformmethanol-water at a ratio (1:1:1) was superior at detecting antibacterial activities and significant quantities of antibacterial compounds. Herein, we demonstrate that antibacterial secondary metabolites are produced from G. boninense mycelia. Antibacterial compounds were monitored in concurrent biochemical and biophysical experiments. The combined methods included high performance thin-layer chromatography (HPTLC), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR) spectroscopy. The antibacterial compounds derived from mycelia with chloroform-methanol extraction through LLE were isolated via a gradient solvent elution system using HPTLC. The antibacterial activity of the isolated compounds was observed to be the most potent against Staphylococcus aureus ATCC 25923 and multidrug-resistant S. aureus NCTC 11939. GC-MS, HPLC, and FTIR analysis confirmed two antibacterial compounds, which were identified as 4,4,14α-trimethylcholestane (m/z = 414.75; lanostane, C(30)H(54)) and ergosta-5,7,22-trien-3β-ol (m/z = 396.65; ergosterol, C(28)H(44)O). With the aid of spectroscopic evaluations, ganoboninketal (m/z = 498.66, C(30)H(42)O(6)), which belongs to the 3,4-seco-27-norlanostane triterpene family, was additionally characterized by 2D-NMR analysis. Despite the lack of antibacterial potential exhibited by lanostane; both ergosterol and ganoboninketal displayed significant antibacterial activities against bacterial pathogens. Results provide evidence for the existence of bioactive compounds in the mycelia of the relatively unexplored phytopathogenic G. boninense, together with a robust method for estimating the corresponding potent antibacterial secondary metabolites. ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available for this article at 10.1007/s12275-021-0551-8 and is accessible for authorized users. The Microbiological Society of Korea 2020-12-23 2021 /pmc/articles/PMC7756191/ /pubmed/33355891 http://dx.doi.org/10.1007/s12275-021-0551-8 Text en © The Microbiological Society of Korea 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Microbial Physiology and Biochemistry
Abdullah, Syahriel
Oh, Yoon Sin
Kwak, Min-Kyu
Chong, KhimPhin
Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense
title Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense
title_full Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense
title_fullStr Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense
title_full_unstemmed Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense
title_short Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense
title_sort biophysical characterization of antibacterial compounds derived from pathogenic fungi ganoderma boninense
topic Microbial Physiology and Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756191/
https://www.ncbi.nlm.nih.gov/pubmed/33355891
http://dx.doi.org/10.1007/s12275-021-0551-8
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