Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells

A new family of robust, non‐toxic, water‐compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity‐sensitive BODIPY fluorop...

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Detalles Bibliográficos
Autores principales: Robson, Jonathan A., Kubánková, Markéta, Bond, Tamzin, Hendley, Rian A., White, Andrew J. P., Kuimova, Marina K., Wilton‐Ely, James D. E. T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756414/
https://www.ncbi.nlm.nih.gov/pubmed/32686308
http://dx.doi.org/10.1002/anie.202008224
Descripción
Sumario:A new family of robust, non‐toxic, water‐compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity‐sensitive BODIPY fluorophore that allows the measurement of microscopic viscosity in live cells via fluorescence lifetime imaging microscopy (FLIM) while also monitoring CO levels. This is the first example of a probe that can simultaneously detect CO alongside small viscosity changes in organelles of live cells.

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