Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells

A new family of robust, non‐toxic, water‐compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity‐sensitive BODIPY fluorop...

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Autores principales: Robson, Jonathan A., Kubánková, Markéta, Bond, Tamzin, Hendley, Rian A., White, Andrew J. P., Kuimova, Marina K., Wilton‐Ely, James D. E. T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756414/
https://www.ncbi.nlm.nih.gov/pubmed/32686308
http://dx.doi.org/10.1002/anie.202008224
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author Robson, Jonathan A.
Kubánková, Markéta
Bond, Tamzin
Hendley, Rian A.
White, Andrew J. P.
Kuimova, Marina K.
Wilton‐Ely, James D. E. T.
author_facet Robson, Jonathan A.
Kubánková, Markéta
Bond, Tamzin
Hendley, Rian A.
White, Andrew J. P.
Kuimova, Marina K.
Wilton‐Ely, James D. E. T.
author_sort Robson, Jonathan A.
collection PubMed
description A new family of robust, non‐toxic, water‐compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity‐sensitive BODIPY fluorophore that allows the measurement of microscopic viscosity in live cells via fluorescence lifetime imaging microscopy (FLIM) while also monitoring CO levels. This is the first example of a probe that can simultaneously detect CO alongside small viscosity changes in organelles of live cells.
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spelling pubmed-77564142020-12-28 Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells Robson, Jonathan A. Kubánková, Markéta Bond, Tamzin Hendley, Rian A. White, Andrew J. P. Kuimova, Marina K. Wilton‐Ely, James D. E. T. Angew Chem Int Ed Engl Communications A new family of robust, non‐toxic, water‐compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity‐sensitive BODIPY fluorophore that allows the measurement of microscopic viscosity in live cells via fluorescence lifetime imaging microscopy (FLIM) while also monitoring CO levels. This is the first example of a probe that can simultaneously detect CO alongside small viscosity changes in organelles of live cells. John Wiley and Sons Inc. 2020-09-17 2020-11-23 /pmc/articles/PMC7756414/ /pubmed/32686308 http://dx.doi.org/10.1002/anie.202008224 Text en © 2020 The Authors. Published by Wiley-VCH GmbH This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Robson, Jonathan A.
Kubánková, Markéta
Bond, Tamzin
Hendley, Rian A.
White, Andrew J. P.
Kuimova, Marina K.
Wilton‐Ely, James D. E. T.
Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells
title Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells
title_full Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells
title_fullStr Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells
title_full_unstemmed Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells
title_short Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells
title_sort simultaneous detection of carbon monoxide and viscosity changes in cells
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756414/
https://www.ncbi.nlm.nih.gov/pubmed/32686308
http://dx.doi.org/10.1002/anie.202008224
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