Would 20 nm Filtered Fetal Bovine Serum-Supplemented Media Support Growth of CHO and HEK-293 Cells?

[Image: see text] Virus safety of fetal bovine serum (FBS) is a critical issue for cell culture and clinical applications of cell therapies. The size exclusion filtration of FBS-supplemented cell culture media through small-size virus retentive filter paper is presented to investigate its effect on...

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Autores principales: Manukyan, Levon, Marinaki, Maria-Eleni, Mihranyan, Albert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756488/
https://www.ncbi.nlm.nih.gov/pubmed/33381749
http://dx.doi.org/10.1021/acsabm.0c01372
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author Manukyan, Levon
Marinaki, Maria-Eleni
Mihranyan, Albert
author_facet Manukyan, Levon
Marinaki, Maria-Eleni
Mihranyan, Albert
author_sort Manukyan, Levon
collection PubMed
description [Image: see text] Virus safety of fetal bovine serum (FBS) is a critical issue for cell culture and clinical applications of cell therapies. The size exclusion filtration of FBS-supplemented cell culture media through small-size virus retentive filter paper is presented to investigate its effect on cell culture. A substantial proportion of proteins (ca. 45%) was removed by nanofiltration, yet important transport proteins (albumin, fetuins, macroglobulins, transferrin) were unaffected. The cell viability of Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK-293) cells that were grown in media supplemented with nanofiltered FBS was surprisingly high, despite the observed protein losses. Protein depletion following nanofiltration resulted in detectable levels of autophagy markers.
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spelling pubmed-77564882020-12-28 Would 20 nm Filtered Fetal Bovine Serum-Supplemented Media Support Growth of CHO and HEK-293 Cells? Manukyan, Levon Marinaki, Maria-Eleni Mihranyan, Albert ACS Appl Bio Mater [Image: see text] Virus safety of fetal bovine serum (FBS) is a critical issue for cell culture and clinical applications of cell therapies. The size exclusion filtration of FBS-supplemented cell culture media through small-size virus retentive filter paper is presented to investigate its effect on cell culture. A substantial proportion of proteins (ca. 45%) was removed by nanofiltration, yet important transport proteins (albumin, fetuins, macroglobulins, transferrin) were unaffected. The cell viability of Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK-293) cells that were grown in media supplemented with nanofiltered FBS was surprisingly high, despite the observed protein losses. Protein depletion following nanofiltration resulted in detectable levels of autophagy markers. American Chemical Society 2020-12-08 2020-12-21 /pmc/articles/PMC7756488/ /pubmed/33381749 http://dx.doi.org/10.1021/acsabm.0c01372 Text en © 2020 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Manukyan, Levon
Marinaki, Maria-Eleni
Mihranyan, Albert
Would 20 nm Filtered Fetal Bovine Serum-Supplemented Media Support Growth of CHO and HEK-293 Cells?
title Would 20 nm Filtered Fetal Bovine Serum-Supplemented Media Support Growth of CHO and HEK-293 Cells?
title_full Would 20 nm Filtered Fetal Bovine Serum-Supplemented Media Support Growth of CHO and HEK-293 Cells?
title_fullStr Would 20 nm Filtered Fetal Bovine Serum-Supplemented Media Support Growth of CHO and HEK-293 Cells?
title_full_unstemmed Would 20 nm Filtered Fetal Bovine Serum-Supplemented Media Support Growth of CHO and HEK-293 Cells?
title_short Would 20 nm Filtered Fetal Bovine Serum-Supplemented Media Support Growth of CHO and HEK-293 Cells?
title_sort would 20 nm filtered fetal bovine serum-supplemented media support growth of cho and hek-293 cells?
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756488/
https://www.ncbi.nlm.nih.gov/pubmed/33381749
http://dx.doi.org/10.1021/acsabm.0c01372
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