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Development of a Reduced‐Volume Acute Lethality Toxicity Test for Hyalella azteca

Effects‐directed analysis (EDA) is used to identify the principal toxic components within a complex mixture using iterative steps of chemical fractionation guided by bioassay results. Bioassay selection can be limited in EDA because of the volume requirements for many standardized test methods, and...

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Autores principales: Rodrigues, Maegan R., Frank, Richard A., Schissler, Daniel M., Deeth, Lorna E., Brown, Lisa R., Hedges, Amanda M., Dixon, D. George, Hewitt, L. Mark, Bartlett, Adrienne J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756806/
https://www.ncbi.nlm.nih.gov/pubmed/32761933
http://dx.doi.org/10.1002/etc.4840
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author Rodrigues, Maegan R.
Frank, Richard A.
Schissler, Daniel M.
Deeth, Lorna E.
Brown, Lisa R.
Hedges, Amanda M.
Dixon, D. George
Hewitt, L. Mark
Bartlett, Adrienne J.
author_facet Rodrigues, Maegan R.
Frank, Richard A.
Schissler, Daniel M.
Deeth, Lorna E.
Brown, Lisa R.
Hedges, Amanda M.
Dixon, D. George
Hewitt, L. Mark
Bartlett, Adrienne J.
author_sort Rodrigues, Maegan R.
collection PubMed
description Effects‐directed analysis (EDA) is used to identify the principal toxic components within a complex mixture using iterative steps of chemical fractionation guided by bioassay results. Bioassay selection can be limited in EDA because of the volume requirements for many standardized test methods, and therefore, a reduced‐volume acute toxicity test that also provides whole‐organism responses is beneficial. To address this need, a static, 7‐d, water‐only, reduced‐volume method (50 mL, 10 organisms) was developed for Hyalella azteca that substantially decreases the volume requirements of standard‐volume acute test exposures (200–500 mL of test solution, 15–20 organisms) while maintaining water quality and meeting control survival criteria. Standard‐ and reduced‐volume methods were compared by conducting concurrent toxicity tests with 2 inorganic toxicants (KCl and CdCl(2)) and 2 organic mixtures of naphthenic acid fraction components (NAFCs) to evaluate test performance. There was no difference between methods when comparing the median lethal concentrations (LC50s) for KCl and both NAFC mixtures (p > 0.05). The LC50s for CdCl(2) were statistically different (p = 0.0002); however, this was not considered biologically meaningful because the difference between LC50s was <2‐fold. In conclusion, the reduced‐volume H. azteca test method generated results comparable to standard‐volume test methods and is suitable for use in situations where limited testing material is available, such as when conducting EDA. Environ Toxicol Chem 2020;39:2221–2227. © 2020 Her Majesty the Queen in Right of Canada. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. Reproduced with the permission of the Minister of Environment and Climate Change Canada.
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spelling pubmed-77568062020-12-28 Development of a Reduced‐Volume Acute Lethality Toxicity Test for Hyalella azteca Rodrigues, Maegan R. Frank, Richard A. Schissler, Daniel M. Deeth, Lorna E. Brown, Lisa R. Hedges, Amanda M. Dixon, D. George Hewitt, L. Mark Bartlett, Adrienne J. Environ Toxicol Chem Environmental Toxicology Effects‐directed analysis (EDA) is used to identify the principal toxic components within a complex mixture using iterative steps of chemical fractionation guided by bioassay results. Bioassay selection can be limited in EDA because of the volume requirements for many standardized test methods, and therefore, a reduced‐volume acute toxicity test that also provides whole‐organism responses is beneficial. To address this need, a static, 7‐d, water‐only, reduced‐volume method (50 mL, 10 organisms) was developed for Hyalella azteca that substantially decreases the volume requirements of standard‐volume acute test exposures (200–500 mL of test solution, 15–20 organisms) while maintaining water quality and meeting control survival criteria. Standard‐ and reduced‐volume methods were compared by conducting concurrent toxicity tests with 2 inorganic toxicants (KCl and CdCl(2)) and 2 organic mixtures of naphthenic acid fraction components (NAFCs) to evaluate test performance. There was no difference between methods when comparing the median lethal concentrations (LC50s) for KCl and both NAFC mixtures (p > 0.05). The LC50s for CdCl(2) were statistically different (p = 0.0002); however, this was not considered biologically meaningful because the difference between LC50s was <2‐fold. In conclusion, the reduced‐volume H. azteca test method generated results comparable to standard‐volume test methods and is suitable for use in situations where limited testing material is available, such as when conducting EDA. Environ Toxicol Chem 2020;39:2221–2227. © 2020 Her Majesty the Queen in Right of Canada. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. Reproduced with the permission of the Minister of Environment and Climate Change Canada. John Wiley and Sons Inc. 2020-09-16 2020-11 /pmc/articles/PMC7756806/ /pubmed/32761933 http://dx.doi.org/10.1002/etc.4840 Text en © 2020 Her Majesty the Queen in Right of Canada. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. Reproduced with the permission of the Minister of Environment and Climate Change Canada This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Environmental Toxicology
Rodrigues, Maegan R.
Frank, Richard A.
Schissler, Daniel M.
Deeth, Lorna E.
Brown, Lisa R.
Hedges, Amanda M.
Dixon, D. George
Hewitt, L. Mark
Bartlett, Adrienne J.
Development of a Reduced‐Volume Acute Lethality Toxicity Test for Hyalella azteca
title Development of a Reduced‐Volume Acute Lethality Toxicity Test for Hyalella azteca
title_full Development of a Reduced‐Volume Acute Lethality Toxicity Test for Hyalella azteca
title_fullStr Development of a Reduced‐Volume Acute Lethality Toxicity Test for Hyalella azteca
title_full_unstemmed Development of a Reduced‐Volume Acute Lethality Toxicity Test for Hyalella azteca
title_short Development of a Reduced‐Volume Acute Lethality Toxicity Test for Hyalella azteca
title_sort development of a reduced‐volume acute lethality toxicity test for hyalella azteca
topic Environmental Toxicology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756806/
https://www.ncbi.nlm.nih.gov/pubmed/32761933
http://dx.doi.org/10.1002/etc.4840
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