Strategies for (1)H‐Detected Dynamic Nuclear Polarization Magic‐Angle Spinning NMR Spectroscopy

Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic‐angle spinning NMR spectroscopy. Herein, the feasibility of proton‐detected experiments with slow (10 kHz) magic angle spinning was demonstrated. The improvement in sensitivity permits the ac...

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Detalles Bibliográficos
Autores principales: Concistré, Maria, Paul, Subhradip, Carravetta, Marina, Kuprov, Ilya, Williamson, Philip T. F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756879/
https://www.ncbi.nlm.nih.gov/pubmed/32827182
http://dx.doi.org/10.1002/chem.202003463
Descripción
Sumario:Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic‐angle spinning NMR spectroscopy. Herein, the feasibility of proton‐detected experiments with slow (10 kHz) magic angle spinning was demonstrated. The improvement in sensitivity permits the acquisition of indirectly detected (14)N NMR spectra allowing biomolecular structures to be characterized without recourse to isotope labelling. This provides a new tool for the structural characterization of environmental and medical samples, in which isotope labelling is frequently intractable.

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