Strategies for (1)H‐Detected Dynamic Nuclear Polarization Magic‐Angle Spinning NMR Spectroscopy

Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic‐angle spinning NMR spectroscopy. Herein, the feasibility of proton‐detected experiments with slow (10 kHz) magic angle spinning was demonstrated. The improvement in sensitivity permits the ac...

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Detalles Bibliográficos
Autores principales: Concistré, Maria, Paul, Subhradip, Carravetta, Marina, Kuprov, Ilya, Williamson, Philip T. F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756879/
https://www.ncbi.nlm.nih.gov/pubmed/32827182
http://dx.doi.org/10.1002/chem.202003463
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author Concistré, Maria
Paul, Subhradip
Carravetta, Marina
Kuprov, Ilya
Williamson, Philip T. F.
author_facet Concistré, Maria
Paul, Subhradip
Carravetta, Marina
Kuprov, Ilya
Williamson, Philip T. F.
author_sort Concistré, Maria
collection PubMed
description Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic‐angle spinning NMR spectroscopy. Herein, the feasibility of proton‐detected experiments with slow (10 kHz) magic angle spinning was demonstrated. The improvement in sensitivity permits the acquisition of indirectly detected (14)N NMR spectra allowing biomolecular structures to be characterized without recourse to isotope labelling. This provides a new tool for the structural characterization of environmental and medical samples, in which isotope labelling is frequently intractable.
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spelling pubmed-77568792020-12-28 Strategies for (1)H‐Detected Dynamic Nuclear Polarization Magic‐Angle Spinning NMR Spectroscopy Concistré, Maria Paul, Subhradip Carravetta, Marina Kuprov, Ilya Williamson, Philip T. F. Chemistry Communications Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic‐angle spinning NMR spectroscopy. Herein, the feasibility of proton‐detected experiments with slow (10 kHz) magic angle spinning was demonstrated. The improvement in sensitivity permits the acquisition of indirectly detected (14)N NMR spectra allowing biomolecular structures to be characterized without recourse to isotope labelling. This provides a new tool for the structural characterization of environmental and medical samples, in which isotope labelling is frequently intractable. John Wiley and Sons Inc. 2020-11-03 2020-12-04 /pmc/articles/PMC7756879/ /pubmed/32827182 http://dx.doi.org/10.1002/chem.202003463 Text en © 2020 The Authors. Published by Wiley-VCH GmbH This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Concistré, Maria
Paul, Subhradip
Carravetta, Marina
Kuprov, Ilya
Williamson, Philip T. F.
Strategies for (1)H‐Detected Dynamic Nuclear Polarization Magic‐Angle Spinning NMR Spectroscopy
title Strategies for (1)H‐Detected Dynamic Nuclear Polarization Magic‐Angle Spinning NMR Spectroscopy
title_full Strategies for (1)H‐Detected Dynamic Nuclear Polarization Magic‐Angle Spinning NMR Spectroscopy
title_fullStr Strategies for (1)H‐Detected Dynamic Nuclear Polarization Magic‐Angle Spinning NMR Spectroscopy
title_full_unstemmed Strategies for (1)H‐Detected Dynamic Nuclear Polarization Magic‐Angle Spinning NMR Spectroscopy
title_short Strategies for (1)H‐Detected Dynamic Nuclear Polarization Magic‐Angle Spinning NMR Spectroscopy
title_sort strategies for (1)h‐detected dynamic nuclear polarization magic‐angle spinning nmr spectroscopy
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756879/
https://www.ncbi.nlm.nih.gov/pubmed/32827182
http://dx.doi.org/10.1002/chem.202003463
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