A Protocol for a Novel Human Ex Vivo Model of Aneurysm

Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present an optimi...

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Detalles Bibliográficos
Autores principales: Bianco, Rosaria, Di Gregoli, Karina, Caputo, Massimo, George, Sarah J., Johnson, Jason L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756922/
https://www.ncbi.nlm.nih.gov/pubmed/33377004
http://dx.doi.org/10.1016/j.xpro.2020.100108
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author Bianco, Rosaria
Di Gregoli, Karina
Caputo, Massimo
George, Sarah J.
Johnson, Jason L.
author_facet Bianco, Rosaria
Di Gregoli, Karina
Caputo, Massimo
George, Sarah J.
Johnson, Jason L.
author_sort Bianco, Rosaria
collection PubMed
description Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present an optimized and validated protocol to isolate human umbilical cord arteries and their subsequent deployment within a bioreactor. Consequently, this reproducible ex vivo human model of aneurysm can be used for pathogenesis studies and accompanying assessment of potential novel therapeutics.
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spelling pubmed-77569222020-12-28 A Protocol for a Novel Human Ex Vivo Model of Aneurysm Bianco, Rosaria Di Gregoli, Karina Caputo, Massimo George, Sarah J. Johnson, Jason L. STAR Protoc Protocol Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present an optimized and validated protocol to isolate human umbilical cord arteries and their subsequent deployment within a bioreactor. Consequently, this reproducible ex vivo human model of aneurysm can be used for pathogenesis studies and accompanying assessment of potential novel therapeutics. Elsevier 2020-09-09 /pmc/articles/PMC7756922/ /pubmed/33377004 http://dx.doi.org/10.1016/j.xpro.2020.100108 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Bianco, Rosaria
Di Gregoli, Karina
Caputo, Massimo
George, Sarah J.
Johnson, Jason L.
A Protocol for a Novel Human Ex Vivo Model of Aneurysm
title A Protocol for a Novel Human Ex Vivo Model of Aneurysm
title_full A Protocol for a Novel Human Ex Vivo Model of Aneurysm
title_fullStr A Protocol for a Novel Human Ex Vivo Model of Aneurysm
title_full_unstemmed A Protocol for a Novel Human Ex Vivo Model of Aneurysm
title_short A Protocol for a Novel Human Ex Vivo Model of Aneurysm
title_sort protocol for a novel human ex vivo model of aneurysm
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756922/
https://www.ncbi.nlm.nih.gov/pubmed/33377004
http://dx.doi.org/10.1016/j.xpro.2020.100108
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