Micropatterning of Cells on Gold Surfaces for Biophysical Applications

We developed a reproducible micropatterning method to manipulate and normalize cell shape and cell-cell separation on gold. We used methoxy polyethylene glycol thiol (PEG-SH) to create a self-assembled monolayer that can be oxidized at desired shapes through a photomask with deep UV light. The oxidi...

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Detalles Bibliográficos
Autores principales: Grandy, Carolin, Kolb, Peter, Port, Fabian, Gottschalk, Kay-Eberhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756938/
https://www.ncbi.nlm.nih.gov/pubmed/33377002
http://dx.doi.org/10.1016/j.xpro.2020.100106
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author Grandy, Carolin
Kolb, Peter
Port, Fabian
Gottschalk, Kay-Eberhard
author_facet Grandy, Carolin
Kolb, Peter
Port, Fabian
Gottschalk, Kay-Eberhard
author_sort Grandy, Carolin
collection PubMed
description We developed a reproducible micropatterning method to manipulate and normalize cell shape and cell-cell separation on gold. We used methoxy polyethylene glycol thiol (PEG-SH) to create a self-assembled monolayer that can be oxidized at desired shapes through a photomask with deep UV light. The oxidized PEG can be coated with extracellular matrix proteins and seeded with cells adopting the pre-defined shape. The developed and analyzed surfaces can be used in a wide range of biophysical applications.
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spelling pubmed-77569382020-12-28 Micropatterning of Cells on Gold Surfaces for Biophysical Applications Grandy, Carolin Kolb, Peter Port, Fabian Gottschalk, Kay-Eberhard STAR Protoc Protocol We developed a reproducible micropatterning method to manipulate and normalize cell shape and cell-cell separation on gold. We used methoxy polyethylene glycol thiol (PEG-SH) to create a self-assembled monolayer that can be oxidized at desired shapes through a photomask with deep UV light. The oxidized PEG can be coated with extracellular matrix proteins and seeded with cells adopting the pre-defined shape. The developed and analyzed surfaces can be used in a wide range of biophysical applications. Elsevier 2020-09-17 /pmc/articles/PMC7756938/ /pubmed/33377002 http://dx.doi.org/10.1016/j.xpro.2020.100106 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Grandy, Carolin
Kolb, Peter
Port, Fabian
Gottschalk, Kay-Eberhard
Micropatterning of Cells on Gold Surfaces for Biophysical Applications
title Micropatterning of Cells on Gold Surfaces for Biophysical Applications
title_full Micropatterning of Cells on Gold Surfaces for Biophysical Applications
title_fullStr Micropatterning of Cells on Gold Surfaces for Biophysical Applications
title_full_unstemmed Micropatterning of Cells on Gold Surfaces for Biophysical Applications
title_short Micropatterning of Cells on Gold Surfaces for Biophysical Applications
title_sort micropatterning of cells on gold surfaces for biophysical applications
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756938/
https://www.ncbi.nlm.nih.gov/pubmed/33377002
http://dx.doi.org/10.1016/j.xpro.2020.100106
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