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ESI mutagenesis: a one-step method for introducing mutations into bacterial artificial chromosomes
Bacterial artificial chromosome (BAC)–based transgenes have emerged as a powerful tool for controlled and conditional interrogation of protein function in higher eukaryotes. Although homologous recombination-based recombineering methods have streamlined the efficient integration of protein tags onto...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756954/ https://www.ncbi.nlm.nih.gov/pubmed/33293335 http://dx.doi.org/10.26508/lsa.202000836 |