Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells

Mouse embryonic stem cells (mESCs) are a powerful model to study early mouse development. These blastocyst-derived cells can maintain pluripotency and differentiate into the three embryonic germ layers and an extraembryonic layer, the extraembryonic endoderm (ExEn), which shares similar molecular ma...

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Autores principales: Ngondo, Richard Patryk, Cohen-Tannoudji, Michel, Ciaudo, Constance
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756973/
https://www.ncbi.nlm.nih.gov/pubmed/33377021
http://dx.doi.org/10.1016/j.xpro.2020.100127
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author Ngondo, Richard Patryk
Cohen-Tannoudji, Michel
Ciaudo, Constance
author_facet Ngondo, Richard Patryk
Cohen-Tannoudji, Michel
Ciaudo, Constance
author_sort Ngondo, Richard Patryk
collection PubMed
description Mouse embryonic stem cells (mESCs) are a powerful model to study early mouse development. These blastocyst-derived cells can maintain pluripotency and differentiate into the three embryonic germ layers and an extraembryonic layer, the extraembryonic endoderm (ExEn), which shares similar molecular markers to the definitive endoderm. Here, we present a fast procedure to identify a differentiation defect of mESCs toward ExEn in vitro through the molecular and cellular characterization of embryoid bodies, followed by direct differentiation of mESCs into ExEn. For complete details on the use and execution of this protocol, please refer to Ngondo et al. (2018).
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spelling pubmed-77569732020-12-28 Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells Ngondo, Richard Patryk Cohen-Tannoudji, Michel Ciaudo, Constance STAR Protoc Protocol Mouse embryonic stem cells (mESCs) are a powerful model to study early mouse development. These blastocyst-derived cells can maintain pluripotency and differentiate into the three embryonic germ layers and an extraembryonic layer, the extraembryonic endoderm (ExEn), which shares similar molecular markers to the definitive endoderm. Here, we present a fast procedure to identify a differentiation defect of mESCs toward ExEn in vitro through the molecular and cellular characterization of embryoid bodies, followed by direct differentiation of mESCs into ExEn. For complete details on the use and execution of this protocol, please refer to Ngondo et al. (2018). Elsevier 2020-10-10 /pmc/articles/PMC7756973/ /pubmed/33377021 http://dx.doi.org/10.1016/j.xpro.2020.100127 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ngondo, Richard Patryk
Cohen-Tannoudji, Michel
Ciaudo, Constance
Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells
title Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells
title_full Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells
title_fullStr Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells
title_full_unstemmed Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells
title_short Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells
title_sort fast in vitro procedure to identify extraembryonic differentiation defect of mouse embryonic stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756973/
https://www.ncbi.nlm.nih.gov/pubmed/33377021
http://dx.doi.org/10.1016/j.xpro.2020.100127
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