Dual RNA-Sequencing of Mycobacterium tuberculosis-Infected Cells from a Murine Infection Model

Dual RNA-sequencing is a powerful technique to assess both bacterial and host transcriptomes in an unbiased way. We developed a protocol to perform Dual RNA-seq on in vivo-derived macrophage populations infected with Mycobacterium tuberculosis. Here, we provide a practical step-by-step guide to exec...

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Detalles Bibliográficos
Autores principales: Pisu, Davide, Huang, Lu, Rin Lee, Bom Nae, Grenier, Jennifer K., Russell, David G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756974/
https://www.ncbi.nlm.nih.gov/pubmed/33377017
http://dx.doi.org/10.1016/j.xpro.2020.100123
Descripción
Sumario:Dual RNA-sequencing is a powerful technique to assess both bacterial and host transcriptomes in an unbiased way. We developed a protocol to perform Dual RNA-seq on in vivo-derived macrophage populations infected with Mycobacterium tuberculosis. Here, we provide a practical step-by-step guide to execute the protocol on Mtb-infected cells from a murine infection model. Our protocol can also be easily applied to perform Dual RNA-seq on in vitro-derived cells as well as different Mtb-infected host cell types. For complete details on the use and execution of this protocol, please refer to Pisu et al. (2020)

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