Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids

Patient-derived organoids maintain functional and phenotypic characteristics of the original tissue such as cell-type diversity. Here, we provide protocols on how to label intestinal (cancer) stem cells by integrating the stem cell ASCL2 reporter (STAR) into human and mouse genomes via two different...

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Detalles Bibliográficos
Autores principales: Heinz, Maria C., Oost, Koen C., Snippert, Hugo J.G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757012/
https://www.ncbi.nlm.nih.gov/pubmed/33377020
http://dx.doi.org/10.1016/j.xpro.2020.100126
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author Heinz, Maria C.
Oost, Koen C.
Snippert, Hugo J.G.
author_facet Heinz, Maria C.
Oost, Koen C.
Snippert, Hugo J.G.
author_sort Heinz, Maria C.
collection PubMed
description Patient-derived organoids maintain functional and phenotypic characteristics of the original tissue such as cell-type diversity. Here, we provide protocols on how to label intestinal (cancer) stem cells by integrating the stem cell ASCL2 reporter (STAR) into human and mouse genomes via two different strategies: (1) lentiviral transduction or (2) transposon-based integration. Organoid technology, in combination with the user-friendly nature of STAR, will facilitate basic research in human and mouse adult stem cell biology. For complete details on the use and execution of this protocol, please refer to Oost et al. (2018).
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spelling pubmed-77570122020-12-28 Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids Heinz, Maria C. Oost, Koen C. Snippert, Hugo J.G. STAR Protoc Protocol Patient-derived organoids maintain functional and phenotypic characteristics of the original tissue such as cell-type diversity. Here, we provide protocols on how to label intestinal (cancer) stem cells by integrating the stem cell ASCL2 reporter (STAR) into human and mouse genomes via two different strategies: (1) lentiviral transduction or (2) transposon-based integration. Organoid technology, in combination with the user-friendly nature of STAR, will facilitate basic research in human and mouse adult stem cell biology. For complete details on the use and execution of this protocol, please refer to Oost et al. (2018). Elsevier 2020-10-08 /pmc/articles/PMC7757012/ /pubmed/33377020 http://dx.doi.org/10.1016/j.xpro.2020.100126 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Heinz, Maria C.
Oost, Koen C.
Snippert, Hugo J.G.
Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids
title Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids
title_full Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids
title_fullStr Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids
title_full_unstemmed Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids
title_short Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids
title_sort introducing the stem cell ascl2 reporter star into intestinal organoids
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757012/
https://www.ncbi.nlm.nih.gov/pubmed/33377020
http://dx.doi.org/10.1016/j.xpro.2020.100126
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