Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets

We present a reproducible protocol to prepare droplet-embedded vesicles (DEVs) consisting of an oil droplet embedded within a phospholipid bilayer. This model system mimics a cellular lipid droplet (LD) in physical contact with the endoplasmic reticulum (ER) bilayer. It has the advantage that the li...

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Detalles Bibliográficos
Autores principales: Chorlay, Aymeric, Santinho, Alexandre, Thiam, Abdou Rachid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757013/
https://www.ncbi.nlm.nih.gov/pubmed/33377012
http://dx.doi.org/10.1016/j.xpro.2020.100116
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author Chorlay, Aymeric
Santinho, Alexandre
Thiam, Abdou Rachid
author_facet Chorlay, Aymeric
Santinho, Alexandre
Thiam, Abdou Rachid
author_sort Chorlay, Aymeric
collection PubMed
description We present a reproducible protocol to prepare droplet-embedded vesicles (DEVs) consisting of an oil droplet embedded within a phospholipid bilayer. This model system mimics a cellular lipid droplet (LD) in physical contact with the endoplasmic reticulum (ER) bilayer. It has the advantage that the lipid composition and the biophysical properties of the droplet and the bilayer are controlled and tunable. DEVs can be used to study LD biogenesis factors and determinants of protein binding between ER and LD interfaces. For complete details on the use and execution of this protocol, please refer to Chorlay and Thiam (2020) and Santinho et al. (2020).
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spelling pubmed-77570132020-12-28 Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets Chorlay, Aymeric Santinho, Alexandre Thiam, Abdou Rachid STAR Protoc Protocol We present a reproducible protocol to prepare droplet-embedded vesicles (DEVs) consisting of an oil droplet embedded within a phospholipid bilayer. This model system mimics a cellular lipid droplet (LD) in physical contact with the endoplasmic reticulum (ER) bilayer. It has the advantage that the lipid composition and the biophysical properties of the droplet and the bilayer are controlled and tunable. DEVs can be used to study LD biogenesis factors and determinants of protein binding between ER and LD interfaces. For complete details on the use and execution of this protocol, please refer to Chorlay and Thiam (2020) and Santinho et al. (2020). Elsevier 2020-09-25 /pmc/articles/PMC7757013/ /pubmed/33377012 http://dx.doi.org/10.1016/j.xpro.2020.100116 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Chorlay, Aymeric
Santinho, Alexandre
Thiam, Abdou Rachid
Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets
title Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets
title_full Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets
title_fullStr Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets
title_full_unstemmed Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets
title_short Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets
title_sort making droplet-embedded vesicles to model cellular lipid droplets
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757013/
https://www.ncbi.nlm.nih.gov/pubmed/33377012
http://dx.doi.org/10.1016/j.xpro.2020.100116
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