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Protocol for Isolation and Characterization of In Situ Fixed Quiescent Muscle Stem Cells

Quiescent muscle stem cells, also called satellite cells (SCs), are essential for muscle regeneration. However, quiescent SCs are quickly activated during fluorescence-activated cell sorting (FACS) isolation. Here, we present an optimized protocol to isolate quiescent muscle stem cells from fixative...

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Detalles Bibliográficos
Autores principales: Yue, Lu, Cheung, Tom H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757111/
https://www.ncbi.nlm.nih.gov/pubmed/33377022
http://dx.doi.org/10.1016/j.xpro.2020.100128