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Cytokine responses to various larval stages of equine strongyles and modulatory effects of the adjuvant G3 in vitro

AIMS: To generate different larval stages of Strongylus vulgaris and to study cytokine responses in cultures of eqPBMC exposed to defined larval stages of S. vulgaris and cyathostomins with the aim to understand the early immune reaction to these parasites. METHODS AND RESULTS: EqPBMC were exposed t...

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Autores principales: Hellman, Stina, Tydén, Eva, Hjertner, Bernt, Nilsfors, Frida, Hu, Kefei, Morein, Bror, Fossum, Caroline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757165/
https://www.ncbi.nlm.nih.gov/pubmed/32969532
http://dx.doi.org/10.1111/pim.12794
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author Hellman, Stina
Tydén, Eva
Hjertner, Bernt
Nilsfors, Frida
Hu, Kefei
Morein, Bror
Fossum, Caroline
author_facet Hellman, Stina
Tydén, Eva
Hjertner, Bernt
Nilsfors, Frida
Hu, Kefei
Morein, Bror
Fossum, Caroline
author_sort Hellman, Stina
collection PubMed
description AIMS: To generate different larval stages of Strongylus vulgaris and to study cytokine responses in cultures of eqPBMC exposed to defined larval stages of S. vulgaris and cyathostomins with the aim to understand the early immune reaction to these parasites. METHODS AND RESULTS: EqPBMC were exposed to S. vulgaris larvae (L3, exsheated L3 and L4) and cyathostomin L3 and analysed for cytokine gene expression. Procedures for decontamination, culturing and attenuation of larvae were established. Transcription of IL‐4, IL‐5 and IL‐13 was induced by both S. vulgaris and cyathostomin L3. Moulting of S. vulgaris from L3 to L4 stage was accompanied by a shift to high expression of IL‐5 and IL‐9 (exsheated L3 and L4) and IFN‐γ (L4 only). In parallel, the adjuvant G3 modified the cytokine profile induced by both parasites by reducing the expression of IL‐4, IL‐5 and IL‐10 while concomitantly enhancing the expression of IFN‐γ. CONCLUSION: The L4 stage of S. vulgaris generated a cytokine profile different from that induced by the earlier L3 stage of S. vulgaris and cyathostomins. This diversity depending on the life cycle stage will have implications for the choice of antigen and adjuvant in future vaccine design.
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spelling pubmed-77571652020-12-28 Cytokine responses to various larval stages of equine strongyles and modulatory effects of the adjuvant G3 in vitro Hellman, Stina Tydén, Eva Hjertner, Bernt Nilsfors, Frida Hu, Kefei Morein, Bror Fossum, Caroline Parasite Immunol Original Papers AIMS: To generate different larval stages of Strongylus vulgaris and to study cytokine responses in cultures of eqPBMC exposed to defined larval stages of S. vulgaris and cyathostomins with the aim to understand the early immune reaction to these parasites. METHODS AND RESULTS: EqPBMC were exposed to S. vulgaris larvae (L3, exsheated L3 and L4) and cyathostomin L3 and analysed for cytokine gene expression. Procedures for decontamination, culturing and attenuation of larvae were established. Transcription of IL‐4, IL‐5 and IL‐13 was induced by both S. vulgaris and cyathostomin L3. Moulting of S. vulgaris from L3 to L4 stage was accompanied by a shift to high expression of IL‐5 and IL‐9 (exsheated L3 and L4) and IFN‐γ (L4 only). In parallel, the adjuvant G3 modified the cytokine profile induced by both parasites by reducing the expression of IL‐4, IL‐5 and IL‐10 while concomitantly enhancing the expression of IFN‐γ. CONCLUSION: The L4 stage of S. vulgaris generated a cytokine profile different from that induced by the earlier L3 stage of S. vulgaris and cyathostomins. This diversity depending on the life cycle stage will have implications for the choice of antigen and adjuvant in future vaccine design. John Wiley and Sons Inc. 2020-10-04 2021-01 /pmc/articles/PMC7757165/ /pubmed/32969532 http://dx.doi.org/10.1111/pim.12794 Text en © 2020 The Authors. Parasite Immunology published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Papers
Hellman, Stina
Tydén, Eva
Hjertner, Bernt
Nilsfors, Frida
Hu, Kefei
Morein, Bror
Fossum, Caroline
Cytokine responses to various larval stages of equine strongyles and modulatory effects of the adjuvant G3 in vitro
title Cytokine responses to various larval stages of equine strongyles and modulatory effects of the adjuvant G3 in vitro
title_full Cytokine responses to various larval stages of equine strongyles and modulatory effects of the adjuvant G3 in vitro
title_fullStr Cytokine responses to various larval stages of equine strongyles and modulatory effects of the adjuvant G3 in vitro
title_full_unstemmed Cytokine responses to various larval stages of equine strongyles and modulatory effects of the adjuvant G3 in vitro
title_short Cytokine responses to various larval stages of equine strongyles and modulatory effects of the adjuvant G3 in vitro
title_sort cytokine responses to various larval stages of equine strongyles and modulatory effects of the adjuvant g3 in vitro
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757165/
https://www.ncbi.nlm.nih.gov/pubmed/32969532
http://dx.doi.org/10.1111/pim.12794
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