Protocol for Patch-Seq of Small Interneurons

Obtaining electrophysiological recordings and gene expression information from the same neuron (Patch-seq) brings forward a unique opportunity to study the transcriptional correlates of functional properties and vice versa. Here, we provide a detailed Patch-seq protocol tailored to the specialized d...

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Detalles Bibliográficos
Autores principales: Lipovsek, Marcela, Browne, Lorcan, Grubb, Matthew S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757288/
https://www.ncbi.nlm.nih.gov/pubmed/33377040
http://dx.doi.org/10.1016/j.xpro.2020.100146
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author Lipovsek, Marcela
Browne, Lorcan
Grubb, Matthew S.
author_facet Lipovsek, Marcela
Browne, Lorcan
Grubb, Matthew S.
author_sort Lipovsek, Marcela
collection PubMed
description Obtaining electrophysiological recordings and gene expression information from the same neuron (Patch-seq) brings forward a unique opportunity to study the transcriptional correlates of functional properties and vice versa. Here, we provide a detailed Patch-seq protocol tailored to the specialized demands of studying small interneurons. Focusing on the technically demanding process of transitioning between patch recordings and cell extraction, our protocol describes and troubleshoots steps for successfully collecting small interneurons, allowing for multi-modal Patch-seq interrogation of this crucial cell type.
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spelling pubmed-77572882020-12-28 Protocol for Patch-Seq of Small Interneurons Lipovsek, Marcela Browne, Lorcan Grubb, Matthew S. STAR Protoc Protocol Obtaining electrophysiological recordings and gene expression information from the same neuron (Patch-seq) brings forward a unique opportunity to study the transcriptional correlates of functional properties and vice versa. Here, we provide a detailed Patch-seq protocol tailored to the specialized demands of studying small interneurons. Focusing on the technically demanding process of transitioning between patch recordings and cell extraction, our protocol describes and troubleshoots steps for successfully collecting small interneurons, allowing for multi-modal Patch-seq interrogation of this crucial cell type. Elsevier 2020-10-22 /pmc/articles/PMC7757288/ /pubmed/33377040 http://dx.doi.org/10.1016/j.xpro.2020.100146 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Lipovsek, Marcela
Browne, Lorcan
Grubb, Matthew S.
Protocol for Patch-Seq of Small Interneurons
title Protocol for Patch-Seq of Small Interneurons
title_full Protocol for Patch-Seq of Small Interneurons
title_fullStr Protocol for Patch-Seq of Small Interneurons
title_full_unstemmed Protocol for Patch-Seq of Small Interneurons
title_short Protocol for Patch-Seq of Small Interneurons
title_sort protocol for patch-seq of small interneurons
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757288/
https://www.ncbi.nlm.nih.gov/pubmed/33377040
http://dx.doi.org/10.1016/j.xpro.2020.100146
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