Single-Molecule Fluorescence Imaging in Living Saccharomyces cerevisiae Cells

This protocol describes how to image fluorescently tagged proteins, RNA, or DNA inside living Saccharomyces cerevisiae cells at the single-molecule level. Imaging inside living cells, as opposed to fixed materials, gives access to real-time kinetic information. Although various single-molecule imagi...

Descripción completa

Detalles Bibliográficos
Autores principales: Brouwer, Ineke, Patel, Heta Piyush, Meeussen, Joseph Victor Willem, Pomp, Wim, Lenstra, Tineke Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757289/
https://www.ncbi.nlm.nih.gov/pubmed/33377036
http://dx.doi.org/10.1016/j.xpro.2020.100142

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757289/
https://www.ncbi.nlm.nih.gov/pubmed/33377036
http://dx.doi.org/10.1016/j.xpro.2020.100142

Ejemplares similares