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Single-Molecule Fluorescence Imaging in Living Saccharomyces cerevisiae Cells

This protocol describes how to image fluorescently tagged proteins, RNA, or DNA inside living Saccharomyces cerevisiae cells at the single-molecule level. Imaging inside living cells, as opposed to fixed materials, gives access to real-time kinetic information. Although various single-molecule imagi...

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Detalles Bibliográficos
Autores principales:	Brouwer, Ineke, Patel, Heta Piyush, Meeussen, Joseph Victor Willem, Pomp, Wim, Lenstra, Tineke Laura
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2020
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757289/ https://www.ncbi.nlm.nih.gov/pubmed/33377036 http://dx.doi.org/10.1016/j.xpro.2020.100142

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