Nonrandom DNA Segregation Detection under Replication Stress

Nonrandom DNA segregation (NDS) is a mitotic event in which sister chromatids carrying the old (parent) DNA strands are distributed exclusively to one of the two daughter cells. Although this phenomenon occurs in multiple organisms, the low frequency poses an obstacle to observation. Here, we presen...

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Autores principales: Chen, Sisi, Ji, Fang, Xing, Meichun, Chen, Gaoying, Li, Jiaqian, Geng, Xinwei, Zhang, Chao, Syeda, Madiha Zahra, Ying, Songmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757299/
https://www.ncbi.nlm.nih.gov/pubmed/33377037
http://dx.doi.org/10.1016/j.xpro.2020.100143
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author Chen, Sisi
Ji, Fang
Xing, Meichun
Chen, Gaoying
Li, Jiaqian
Geng, Xinwei
Zhang, Chao
Syeda, Madiha Zahra
Ying, Songmin
author_facet Chen, Sisi
Ji, Fang
Xing, Meichun
Chen, Gaoying
Li, Jiaqian
Geng, Xinwei
Zhang, Chao
Syeda, Madiha Zahra
Ying, Songmin
author_sort Chen, Sisi
collection PubMed
description Nonrandom DNA segregation (NDS) is a mitotic event in which sister chromatids carrying the old (parent) DNA strands are distributed exclusively to one of the two daughter cells. Although this phenomenon occurs in multiple organisms, the low frequency poses an obstacle to observation. Here, we present an improved protocol to induce NDS under replication stress. This protocol can be modified to accommodate various cell lines. For complete details on the use and execution of this protocol, please refer to Xing et al. (2020).
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spelling pubmed-77572992020-12-28 Nonrandom DNA Segregation Detection under Replication Stress Chen, Sisi Ji, Fang Xing, Meichun Chen, Gaoying Li, Jiaqian Geng, Xinwei Zhang, Chao Syeda, Madiha Zahra Ying, Songmin STAR Protoc Protocol Nonrandom DNA segregation (NDS) is a mitotic event in which sister chromatids carrying the old (parent) DNA strands are distributed exclusively to one of the two daughter cells. Although this phenomenon occurs in multiple organisms, the low frequency poses an obstacle to observation. Here, we present an improved protocol to induce NDS under replication stress. This protocol can be modified to accommodate various cell lines. For complete details on the use and execution of this protocol, please refer to Xing et al. (2020). Elsevier 2020-10-23 /pmc/articles/PMC7757299/ /pubmed/33377037 http://dx.doi.org/10.1016/j.xpro.2020.100143 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Chen, Sisi
Ji, Fang
Xing, Meichun
Chen, Gaoying
Li, Jiaqian
Geng, Xinwei
Zhang, Chao
Syeda, Madiha Zahra
Ying, Songmin
Nonrandom DNA Segregation Detection under Replication Stress
title Nonrandom DNA Segregation Detection under Replication Stress
title_full Nonrandom DNA Segregation Detection under Replication Stress
title_fullStr Nonrandom DNA Segregation Detection under Replication Stress
title_full_unstemmed Nonrandom DNA Segregation Detection under Replication Stress
title_short Nonrandom DNA Segregation Detection under Replication Stress
title_sort nonrandom dna segregation detection under replication stress
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757299/
https://www.ncbi.nlm.nih.gov/pubmed/33377037
http://dx.doi.org/10.1016/j.xpro.2020.100143
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