Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2

Since its discovery, several ligands of the ZZ domain have been identified; however, molecular and structural information underlying binding of these ligands remains limited. Here, we describe a protocol for biochemical and structural analysis of the ZZ domain of human E3 ubiquitin ligase HERC2 (HER...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Jiuyang, Xue, Zhaoyu, Vann, Kendra R., Shi, Xiaobing, Kutateladze, Tatiana G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757301/
https://www.ncbi.nlm.nih.gov/pubmed/33377049
http://dx.doi.org/10.1016/j.xpro.2020.100155
_version_ 1783626722925608960
author Liu, Jiuyang
Xue, Zhaoyu
Vann, Kendra R.
Shi, Xiaobing
Kutateladze, Tatiana G.
author_facet Liu, Jiuyang
Xue, Zhaoyu
Vann, Kendra R.
Shi, Xiaobing
Kutateladze, Tatiana G.
author_sort Liu, Jiuyang
collection PubMed
description Since its discovery, several ligands of the ZZ domain have been identified; however, molecular and structural information underlying binding of these ligands remains limited. Here, we describe a protocol for biochemical and structural analysis of the ZZ domain of human E3 ubiquitin ligase HERC2 (HERC2(ZZ)) and its interaction with its ligands: the N-terminal tails of histone H3 and SUMO1. This methodology could be applied for characterization of binding activities of other histone readers. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).
format Online
Article
Text
id pubmed-7757301
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-77573012020-12-28 Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2 Liu, Jiuyang Xue, Zhaoyu Vann, Kendra R. Shi, Xiaobing Kutateladze, Tatiana G. STAR Protoc Protocol Since its discovery, several ligands of the ZZ domain have been identified; however, molecular and structural information underlying binding of these ligands remains limited. Here, we describe a protocol for biochemical and structural analysis of the ZZ domain of human E3 ubiquitin ligase HERC2 (HERC2(ZZ)) and its interaction with its ligands: the N-terminal tails of histone H3 and SUMO1. This methodology could be applied for characterization of binding activities of other histone readers. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020). Elsevier 2020-10-28 /pmc/articles/PMC7757301/ /pubmed/33377049 http://dx.doi.org/10.1016/j.xpro.2020.100155 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Liu, Jiuyang
Xue, Zhaoyu
Vann, Kendra R.
Shi, Xiaobing
Kutateladze, Tatiana G.
Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2
title Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2
title_full Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2
title_fullStr Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2
title_full_unstemmed Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2
title_short Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2
title_sort protocol for biochemical analysis and structure determination of the zz domain of the e3 ubiquitin ligase herc2
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757301/
https://www.ncbi.nlm.nih.gov/pubmed/33377049
http://dx.doi.org/10.1016/j.xpro.2020.100155
work_keys_str_mv AT liujiuyang protocolforbiochemicalanalysisandstructuredeterminationofthezzdomainofthee3ubiquitinligaseherc2
AT xuezhaoyu protocolforbiochemicalanalysisandstructuredeterminationofthezzdomainofthee3ubiquitinligaseherc2
AT vannkendrar protocolforbiochemicalanalysisandstructuredeterminationofthezzdomainofthee3ubiquitinligaseherc2
AT shixiaobing protocolforbiochemicalanalysisandstructuredeterminationofthezzdomainofthee3ubiquitinligaseherc2
AT kutateladzetatianag protocolforbiochemicalanalysisandstructuredeterminationofthezzdomainofthee3ubiquitinligaseherc2

Ejemplares similares