Affinity Purification of Label-free Tubulins from Xenopus Egg Extracts

Cytoplasmic extracts from unfertilized Xenopus eggs have made important contributions to our understanding of microtubule dynamics, spindle assembly, and scaling. Until recently, these in vitro studies relied on the use of heterologous tubulin. This protocol allows for the purification of physiologi...

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Detalles Bibliográficos
Autores principales: Reusch, Sebastian, Biswas, Abin, Hirst, William Graham, Reber, Simone
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757314/
https://www.ncbi.nlm.nih.gov/pubmed/33377045
http://dx.doi.org/10.1016/j.xpro.2020.100151

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757314/
https://www.ncbi.nlm.nih.gov/pubmed/33377045
http://dx.doi.org/10.1016/j.xpro.2020.100151

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