Identification and Functional Analysis of Human CD56(neg) NK Cells by Flow Cytometry

Although scarce in the peripheral blood of healthy people, CD56(neg) NK cells are known to be expanded in some pathological conditions. However, studies on CD56(neg) NK cells had revealed contradictions, probably due to the lack of a specific NK cell surface marker that helps to identify this subset...

Descripción completa

Detalles Bibliográficos
Autores principales: Orrantia, Ane, Terrén, Iñigo, Vitallé, Joana, Astarloa-Pando, Gabirel, Zenarruzabeitia, Olatz, Borrego, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757323/
https://www.ncbi.nlm.nih.gov/pubmed/33377043
http://dx.doi.org/10.1016/j.xpro.2020.100149
Descripción
Sumario:Although scarce in the peripheral blood of healthy people, CD56(neg) NK cells are known to be expanded in some pathological conditions. However, studies on CD56(neg) NK cells had revealed contradictions, probably due to the lack of a specific NK cell surface marker that helps to identify this subset. This protocol details the step-by-step procedure for the identification and functional analysis of CD56(neg) NK cells, providing an improved gating strategy for the selection of this intriguing population. For complete details on the use and execution of this protocol, please refer to Orrantia et al. (2020).

Ejemplares similares