Identification and Functional Analysis of Human CD56(neg) NK Cells by Flow Cytometry

Although scarce in the peripheral blood of healthy people, CD56(neg) NK cells are known to be expanded in some pathological conditions. However, studies on CD56(neg) NK cells had revealed contradictions, probably due to the lack of a specific NK cell surface marker that helps to identify this subset...

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Autores principales: Orrantia, Ane, Terrén, Iñigo, Vitallé, Joana, Astarloa-Pando, Gabirel, Zenarruzabeitia, Olatz, Borrego, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757323/
https://www.ncbi.nlm.nih.gov/pubmed/33377043
http://dx.doi.org/10.1016/j.xpro.2020.100149
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author Orrantia, Ane
Terrén, Iñigo
Vitallé, Joana
Astarloa-Pando, Gabirel
Zenarruzabeitia, Olatz
Borrego, Francisco
author_facet Orrantia, Ane
Terrén, Iñigo
Vitallé, Joana
Astarloa-Pando, Gabirel
Zenarruzabeitia, Olatz
Borrego, Francisco
author_sort Orrantia, Ane
collection PubMed
description Although scarce in the peripheral blood of healthy people, CD56(neg) NK cells are known to be expanded in some pathological conditions. However, studies on CD56(neg) NK cells had revealed contradictions, probably due to the lack of a specific NK cell surface marker that helps to identify this subset. This protocol details the step-by-step procedure for the identification and functional analysis of CD56(neg) NK cells, providing an improved gating strategy for the selection of this intriguing population. For complete details on the use and execution of this protocol, please refer to Orrantia et al. (2020).
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spelling pubmed-77573232020-12-28 Identification and Functional Analysis of Human CD56(neg) NK Cells by Flow Cytometry Orrantia, Ane Terrén, Iñigo Vitallé, Joana Astarloa-Pando, Gabirel Zenarruzabeitia, Olatz Borrego, Francisco STAR Protoc Protocol Although scarce in the peripheral blood of healthy people, CD56(neg) NK cells are known to be expanded in some pathological conditions. However, studies on CD56(neg) NK cells had revealed contradictions, probably due to the lack of a specific NK cell surface marker that helps to identify this subset. This protocol details the step-by-step procedure for the identification and functional analysis of CD56(neg) NK cells, providing an improved gating strategy for the selection of this intriguing population. For complete details on the use and execution of this protocol, please refer to Orrantia et al. (2020). Elsevier 2020-10-29 /pmc/articles/PMC7757323/ /pubmed/33377043 http://dx.doi.org/10.1016/j.xpro.2020.100149 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Orrantia, Ane
Terrén, Iñigo
Vitallé, Joana
Astarloa-Pando, Gabirel
Zenarruzabeitia, Olatz
Borrego, Francisco
Identification and Functional Analysis of Human CD56(neg) NK Cells by Flow Cytometry
title Identification and Functional Analysis of Human CD56(neg) NK Cells by Flow Cytometry
title_full Identification and Functional Analysis of Human CD56(neg) NK Cells by Flow Cytometry
title_fullStr Identification and Functional Analysis of Human CD56(neg) NK Cells by Flow Cytometry
title_full_unstemmed Identification and Functional Analysis of Human CD56(neg) NK Cells by Flow Cytometry
title_short Identification and Functional Analysis of Human CD56(neg) NK Cells by Flow Cytometry
title_sort identification and functional analysis of human cd56(neg) nk cells by flow cytometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757323/
https://www.ncbi.nlm.nih.gov/pubmed/33377043
http://dx.doi.org/10.1016/j.xpro.2020.100149
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