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Phenotypic Characterization of SLe(x)+ and CLA+ CD4+ T Cells

Recent advances in high-resolution multiparametric flow cytometry enable ever deeper analysis of human lymphocyte subsets that require rigorous methodology development and optimization. Here, we detail methods to characterize glycosylated Sialyl-Lewis(X) (SLe(X))- or cutaneous lymphocyte-associated...

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Detalles Bibliográficos
Autores principales: Kuri-Cervantes, Leticia, Pampena, Maria Betina, Betts, Michael R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757334/
https://www.ncbi.nlm.nih.gov/pubmed/33377048
http://dx.doi.org/10.1016/j.xpro.2020.100154
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author Kuri-Cervantes, Leticia
Pampena, Maria Betina
Betts, Michael R.
author_facet Kuri-Cervantes, Leticia
Pampena, Maria Betina
Betts, Michael R.
author_sort Kuri-Cervantes, Leticia
collection PubMed
description Recent advances in high-resolution multiparametric flow cytometry enable ever deeper analysis of human lymphocyte subsets that require rigorous methodology development and optimization. Here, we detail methods to characterize glycosylated Sialyl-Lewis(X) (SLe(X))- or cutaneous lymphocyte-associated antigen (CLA)-expressing CD4+ T cells using two separate multiparametric flow cytometry panels enabling the identification of memory subsets, Th subsets, and expression of diverse activation markers and chemokine receptors. The proposed protocol allows optimal resolution of the measured parameters while minimizing background in a 25-parameter experiment. For complete details on the use and execution of this protocol, please refer to Colomb et al. (2020).
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spelling pubmed-77573342020-12-28 Phenotypic Characterization of SLe(x)+ and CLA+ CD4+ T Cells Kuri-Cervantes, Leticia Pampena, Maria Betina Betts, Michael R. STAR Protoc Protocol Recent advances in high-resolution multiparametric flow cytometry enable ever deeper analysis of human lymphocyte subsets that require rigorous methodology development and optimization. Here, we detail methods to characterize glycosylated Sialyl-Lewis(X) (SLe(X))- or cutaneous lymphocyte-associated antigen (CLA)-expressing CD4+ T cells using two separate multiparametric flow cytometry panels enabling the identification of memory subsets, Th subsets, and expression of diverse activation markers and chemokine receptors. The proposed protocol allows optimal resolution of the measured parameters while minimizing background in a 25-parameter experiment. For complete details on the use and execution of this protocol, please refer to Colomb et al. (2020). Elsevier 2020-10-29 /pmc/articles/PMC7757334/ /pubmed/33377048 http://dx.doi.org/10.1016/j.xpro.2020.100154 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kuri-Cervantes, Leticia
Pampena, Maria Betina
Betts, Michael R.
Phenotypic Characterization of SLe(x)+ and CLA+ CD4+ T Cells
title Phenotypic Characterization of SLe(x)+ and CLA+ CD4+ T Cells
title_full Phenotypic Characterization of SLe(x)+ and CLA+ CD4+ T Cells
title_fullStr Phenotypic Characterization of SLe(x)+ and CLA+ CD4+ T Cells
title_full_unstemmed Phenotypic Characterization of SLe(x)+ and CLA+ CD4+ T Cells
title_short Phenotypic Characterization of SLe(x)+ and CLA+ CD4+ T Cells
title_sort phenotypic characterization of sle(x)+ and cla+ cd4+ t cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757334/
https://www.ncbi.nlm.nih.gov/pubmed/33377048
http://dx.doi.org/10.1016/j.xpro.2020.100154
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