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Organoid Sample Preparation and Extraction for LC-MS Peptidomics

This protocol describes the peptidomic analysis of organoid lysates, FACS-purified cell populations, and 2D culture secretions by liquid chromatography mass spectrometry (LC-MS). Currently, most peptides are quantified by ELISA, limiting the peptides that can be studied. However, an LC-MS-based appr...

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Detalles Bibliográficos
Autores principales:	Miedzybrodzka, Emily L., Foreman, Rachel E., Galvin, Sam G., Larraufie, Pierre, George, Amy L., Goldspink, Deborah A., Reimann, Frank, Gribble, Fiona M., Kay, Richard G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2020
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757358/ https://www.ncbi.nlm.nih.gov/pubmed/33377058 http://dx.doi.org/10.1016/j.xpro.2020.100164

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