Establishment of a Long-Term Co-culture Assay for Mesenchymal Stromal Cells and Hematopoietic Stem/Progenitors

We describe a protocol for a long-term co-culture assay to study the contribution of mesenchymal stromal cells (MSCs) in regulating hematopoietic stem/progenitor cell (HSPC) activity. In addition, we describe the use of a clonogenic assay to determine myelo-erythroid differentiation. This long-term...

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Autores principales: Sinha, Sayantani, Chakraborty, Sayan, Sengupta, Amitava
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757360/
https://www.ncbi.nlm.nih.gov/pubmed/33377055
http://dx.doi.org/10.1016/j.xpro.2020.100161
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author Sinha, Sayantani
Chakraborty, Sayan
Sengupta, Amitava
author_facet Sinha, Sayantani
Chakraborty, Sayan
Sengupta, Amitava
author_sort Sinha, Sayantani
collection PubMed
description We describe a protocol for a long-term co-culture assay to study the contribution of mesenchymal stromal cells (MSCs) in regulating hematopoietic stem/progenitor cell (HSPC) activity. In addition, we describe the use of a clonogenic assay to determine myelo-erythroid differentiation. This long-term culture-initiating cell assay can be used for qualitative analysis of MSCs capable of supporting hematopoiesis and may also be used as a proxy readout to study HSPC repopulation. For complete details on the use and execution of this protocol, please refer to Sinha et al. (2020).
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spelling pubmed-77573602020-12-28 Establishment of a Long-Term Co-culture Assay for Mesenchymal Stromal Cells and Hematopoietic Stem/Progenitors Sinha, Sayantani Chakraborty, Sayan Sengupta, Amitava STAR Protoc Protocol We describe a protocol for a long-term co-culture assay to study the contribution of mesenchymal stromal cells (MSCs) in regulating hematopoietic stem/progenitor cell (HSPC) activity. In addition, we describe the use of a clonogenic assay to determine myelo-erythroid differentiation. This long-term culture-initiating cell assay can be used for qualitative analysis of MSCs capable of supporting hematopoiesis and may also be used as a proxy readout to study HSPC repopulation. For complete details on the use and execution of this protocol, please refer to Sinha et al. (2020). Elsevier 2020-11-24 /pmc/articles/PMC7757360/ /pubmed/33377055 http://dx.doi.org/10.1016/j.xpro.2020.100161 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sinha, Sayantani
Chakraborty, Sayan
Sengupta, Amitava
Establishment of a Long-Term Co-culture Assay for Mesenchymal Stromal Cells and Hematopoietic Stem/Progenitors
title Establishment of a Long-Term Co-culture Assay for Mesenchymal Stromal Cells and Hematopoietic Stem/Progenitors
title_full Establishment of a Long-Term Co-culture Assay for Mesenchymal Stromal Cells and Hematopoietic Stem/Progenitors
title_fullStr Establishment of a Long-Term Co-culture Assay for Mesenchymal Stromal Cells and Hematopoietic Stem/Progenitors
title_full_unstemmed Establishment of a Long-Term Co-culture Assay for Mesenchymal Stromal Cells and Hematopoietic Stem/Progenitors
title_short Establishment of a Long-Term Co-culture Assay for Mesenchymal Stromal Cells and Hematopoietic Stem/Progenitors
title_sort establishment of a long-term co-culture assay for mesenchymal stromal cells and hematopoietic stem/progenitors
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757360/
https://www.ncbi.nlm.nih.gov/pubmed/33377055
http://dx.doi.org/10.1016/j.xpro.2020.100161
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