Protocol for Monitoring DNA-Triggered cGAS/STING Signaling in Mammalian Cells and Mice

Innate immunity is the first layer of defense against infection in mammals and is tightly regulated. We monitored cGAS/STING signaling upon ISD90 or 2′,3′-cGAMP stimulation in EA.hy926 cells by western blotting, RT-PCR, and ELISA analyses to reveal signaling activation and IFNβ production. In additi...

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Detalles Bibliográficos
Autores principales: Wang, Ying, Ma, Zhe, Damania, Blossom, Liu, Pengda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757363/
https://www.ncbi.nlm.nih.gov/pubmed/33377065
http://dx.doi.org/10.1016/j.xpro.2020.100171

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