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Preclinical Testing of CAR T Cells in a Patient-Derived Xenograft Model of Glioblastoma
Glioblastoma (GBM) is the most common malignant adult brain tumor that is resistant to the standard care therapy. Advances in chimeric antigen receptor (CAR) T cell therapies have spurred renewed interest in developing CAR T cell therapies to target chemoradiotherapy-resistant brain tumor-initiating...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757398/ https://www.ncbi.nlm.nih.gov/pubmed/33377068 http://dx.doi.org/10.1016/j.xpro.2020.100174 |
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author | Tatari, Nazanin Maich, William T. Salim, Sabra K. Mckenna, Dillon Venugopal, Chitra Singh, Sheila |
author_facet | Tatari, Nazanin Maich, William T. Salim, Sabra K. Mckenna, Dillon Venugopal, Chitra Singh, Sheila |
author_sort | Tatari, Nazanin |
collection | PubMed |
description | Glioblastoma (GBM) is the most common malignant adult brain tumor that is resistant to the standard care therapy. Advances in chimeric antigen receptor (CAR) T cell therapies have spurred renewed interest in developing CAR T cell therapies to target chemoradiotherapy-resistant brain tumor-initiating cells. This protocol shows how to isolate peripheral blood mononuclear cells from healthy donors and generate CAR T cells for the antigens of interest, and how to intracranially inject the CAR T cells into a patient-derived xenograft mouse model of GBM. For complete details on the use and execution of this protocol, please refer to Vora et al. (2020). |
format | Online Article Text |
id | pubmed-7757398 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-77573982020-12-28 Preclinical Testing of CAR T Cells in a Patient-Derived Xenograft Model of Glioblastoma Tatari, Nazanin Maich, William T. Salim, Sabra K. Mckenna, Dillon Venugopal, Chitra Singh, Sheila STAR Protoc Protocol Glioblastoma (GBM) is the most common malignant adult brain tumor that is resistant to the standard care therapy. Advances in chimeric antigen receptor (CAR) T cell therapies have spurred renewed interest in developing CAR T cell therapies to target chemoradiotherapy-resistant brain tumor-initiating cells. This protocol shows how to isolate peripheral blood mononuclear cells from healthy donors and generate CAR T cells for the antigens of interest, and how to intracranially inject the CAR T cells into a patient-derived xenograft mouse model of GBM. For complete details on the use and execution of this protocol, please refer to Vora et al. (2020). Elsevier 2020-12-02 /pmc/articles/PMC7757398/ /pubmed/33377068 http://dx.doi.org/10.1016/j.xpro.2020.100174 Text en Crown Copyright © 2020. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Tatari, Nazanin Maich, William T. Salim, Sabra K. Mckenna, Dillon Venugopal, Chitra Singh, Sheila Preclinical Testing of CAR T Cells in a Patient-Derived Xenograft Model of Glioblastoma |
title | Preclinical Testing of CAR T Cells in a Patient-Derived Xenograft Model of Glioblastoma |
title_full | Preclinical Testing of CAR T Cells in a Patient-Derived Xenograft Model of Glioblastoma |
title_fullStr | Preclinical Testing of CAR T Cells in a Patient-Derived Xenograft Model of Glioblastoma |
title_full_unstemmed | Preclinical Testing of CAR T Cells in a Patient-Derived Xenograft Model of Glioblastoma |
title_short | Preclinical Testing of CAR T Cells in a Patient-Derived Xenograft Model of Glioblastoma |
title_sort | preclinical testing of car t cells in a patient-derived xenograft model of glioblastoma |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757398/ https://www.ncbi.nlm.nih.gov/pubmed/33377068 http://dx.doi.org/10.1016/j.xpro.2020.100174 |
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