Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs

Cell reprogramming has revolutionized the fields of cell and regenerative biology. However, human induced pluripotent stem cell (iPSC) derivation remains inefficient and variable. Here, we present a protocol that uses human menstrual blood-derived stromal cells (MnSCs), which are susceptible to repr...

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Autores principales: Sanzhez-Mata, Alicia, Ferez-Gomez, Alberto, Gonzalez-Muñoz, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757400/
https://www.ncbi.nlm.nih.gov/pubmed/33377077
http://dx.doi.org/10.1016/j.xpro.2020.100183
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author Sanzhez-Mata, Alicia
Ferez-Gomez, Alberto
Gonzalez-Muñoz, Elena
author_facet Sanzhez-Mata, Alicia
Ferez-Gomez, Alberto
Gonzalez-Muñoz, Elena
author_sort Sanzhez-Mata, Alicia
collection PubMed
description Cell reprogramming has revolutionized the fields of cell and regenerative biology. However, human induced pluripotent stem cell (iPSC) derivation remains inefficient and variable. Here, we present a protocol that uses human menstrual blood-derived stromal cells (MnSCs), which are susceptible to reprogramming, as a source of somatic cells. We describe an oocyte-based reprogramming combination to generate AOX15-iPSCs that can be used to study different states of pluripotency. For complete details on the use and execution of this protocol, please refer to Lopez-Caraballo et al. (2020).
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spelling pubmed-77574002020-12-28 Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs Sanzhez-Mata, Alicia Ferez-Gomez, Alberto Gonzalez-Muñoz, Elena STAR Protoc Protocol Cell reprogramming has revolutionized the fields of cell and regenerative biology. However, human induced pluripotent stem cell (iPSC) derivation remains inefficient and variable. Here, we present a protocol that uses human menstrual blood-derived stromal cells (MnSCs), which are susceptible to reprogramming, as a source of somatic cells. We describe an oocyte-based reprogramming combination to generate AOX15-iPSCs that can be used to study different states of pluripotency. For complete details on the use and execution of this protocol, please refer to Lopez-Caraballo et al. (2020). Elsevier 2020-11-25 /pmc/articles/PMC7757400/ /pubmed/33377077 http://dx.doi.org/10.1016/j.xpro.2020.100183 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sanzhez-Mata, Alicia
Ferez-Gomez, Alberto
Gonzalez-Muñoz, Elena
Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs
title Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs
title_full Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs
title_fullStr Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs
title_full_unstemmed Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs
title_short Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs
title_sort protocol to reprogram human menstrual blood-derived stromal cells to generate aox15-ipscs
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757400/
https://www.ncbi.nlm.nih.gov/pubmed/33377077
http://dx.doi.org/10.1016/j.xpro.2020.100183
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