Protocol to Induce Wounding and Measure Membrane Repair in Caenorhabditis elegans Epidermis

Efficient membrane repair after injury is essential for cell and animal survival. Caenorhabditis elegans epidermal cell hpy7 has emerged as a powerful genetic system to investigate the molecular mechanism of membrane repair in vivo. This protocol describes detailed approaches for how to perform woun...

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Detalles Bibliográficos
Autores principales: Wijaya, Chandra Sugiarto, Meng, Xinan, Yang, Qingxian, Xu, Suhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757402/
https://www.ncbi.nlm.nih.gov/pubmed/33377069
http://dx.doi.org/10.1016/j.xpro.2020.100175
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author Wijaya, Chandra Sugiarto
Meng, Xinan
Yang, Qingxian
Xu, Suhong
author_facet Wijaya, Chandra Sugiarto
Meng, Xinan
Yang, Qingxian
Xu, Suhong
author_sort Wijaya, Chandra Sugiarto
collection PubMed
description Efficient membrane repair after injury is essential for cell and animal survival. Caenorhabditis elegans epidermal cell hpy7 has emerged as a powerful genetic system to investigate the molecular mechanism of membrane repair in vivo. This protocol describes detailed approaches for how to perform wounding on the epidermis and how to examine membrane repair by trypan blue staining, confocal imaging, and data analysis. For details on the use and execution of this protocol, please refer to Meng et al. (2020).
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spelling pubmed-77574022020-12-28 Protocol to Induce Wounding and Measure Membrane Repair in Caenorhabditis elegans Epidermis Wijaya, Chandra Sugiarto Meng, Xinan Yang, Qingxian Xu, Suhong STAR Protoc Protocol Efficient membrane repair after injury is essential for cell and animal survival. Caenorhabditis elegans epidermal cell hpy7 has emerged as a powerful genetic system to investigate the molecular mechanism of membrane repair in vivo. This protocol describes detailed approaches for how to perform wounding on the epidermis and how to examine membrane repair by trypan blue staining, confocal imaging, and data analysis. For details on the use and execution of this protocol, please refer to Meng et al. (2020). Elsevier 2020-11-21 /pmc/articles/PMC7757402/ /pubmed/33377069 http://dx.doi.org/10.1016/j.xpro.2020.100175 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Wijaya, Chandra Sugiarto
Meng, Xinan
Yang, Qingxian
Xu, Suhong
Protocol to Induce Wounding and Measure Membrane Repair in Caenorhabditis elegans Epidermis
title Protocol to Induce Wounding and Measure Membrane Repair in Caenorhabditis elegans Epidermis
title_full Protocol to Induce Wounding and Measure Membrane Repair in Caenorhabditis elegans Epidermis
title_fullStr Protocol to Induce Wounding and Measure Membrane Repair in Caenorhabditis elegans Epidermis
title_full_unstemmed Protocol to Induce Wounding and Measure Membrane Repair in Caenorhabditis elegans Epidermis
title_short Protocol to Induce Wounding and Measure Membrane Repair in Caenorhabditis elegans Epidermis
title_sort protocol to induce wounding and measure membrane repair in caenorhabditis elegans epidermis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757402/
https://www.ncbi.nlm.nih.gov/pubmed/33377069
http://dx.doi.org/10.1016/j.xpro.2020.100175
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