Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution

Asymmetric histone inheritance can regulate cell-fate determination in Drosophila male germline stem cells. However, it remains elusive how this mechanism may be used in mammalian system. Recently, we show mouse embryonic stem cells (mESCs) with Wnt3a beads display non-overlapping H3/H4 patterns. He...

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Detalles Bibliográficos
Autores principales: Ma, Binbin, Trieu, Tung-Jui, Habib, Shukry J., Chen, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757403/
https://www.ncbi.nlm.nih.gov/pubmed/33377072
http://dx.doi.org/10.1016/j.xpro.2020.100178
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author Ma, Binbin
Trieu, Tung-Jui
Habib, Shukry J.
Chen, Xin
author_facet Ma, Binbin
Trieu, Tung-Jui
Habib, Shukry J.
Chen, Xin
author_sort Ma, Binbin
collection PubMed
description Asymmetric histone inheritance can regulate cell-fate determination in Drosophila male germline stem cells. However, it remains elusive how this mechanism may be used in mammalian system. Recently, we show mouse embryonic stem cells (mESCs) with Wnt3a beads display non-overlapping H3/H4 patterns. Here, we present a detailed protocol for tracking histone inheritance in asymmetrically dividing mESCs at single-cell resolution. This protocol will establish a new system to study histone inheritance in cultured mammalian cells and could be applied to other parallel systems. For complete details on the use and execution of this protocol, please refer to Tran et al. (2012), Habib et al. (2013), Lowndes et al. (2017), and Ma et al. (2020).
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spelling pubmed-77574032020-12-28 Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution Ma, Binbin Trieu, Tung-Jui Habib, Shukry J. Chen, Xin STAR Protoc Protocol Asymmetric histone inheritance can regulate cell-fate determination in Drosophila male germline stem cells. However, it remains elusive how this mechanism may be used in mammalian system. Recently, we show mouse embryonic stem cells (mESCs) with Wnt3a beads display non-overlapping H3/H4 patterns. Here, we present a detailed protocol for tracking histone inheritance in asymmetrically dividing mESCs at single-cell resolution. This protocol will establish a new system to study histone inheritance in cultured mammalian cells and could be applied to other parallel systems. For complete details on the use and execution of this protocol, please refer to Tran et al. (2012), Habib et al. (2013), Lowndes et al. (2017), and Ma et al. (2020). Elsevier 2020-11-18 /pmc/articles/PMC7757403/ /pubmed/33377072 http://dx.doi.org/10.1016/j.xpro.2020.100178 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ma, Binbin
Trieu, Tung-Jui
Habib, Shukry J.
Chen, Xin
Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution
title Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution
title_full Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution
title_fullStr Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution
title_full_unstemmed Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution
title_short Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution
title_sort protocol for establishing mouse embryonic stem cells to study histone inheritance pattern at single-cell resolution
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757403/
https://www.ncbi.nlm.nih.gov/pubmed/33377072
http://dx.doi.org/10.1016/j.xpro.2020.100178
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