Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen

This protocol describes the comparative proteomic profiling of the spleen of wild type versus mdx-4cv mouse, a model of dystrophinopathy. We detail sample preparation for bottom-up proteomic mass spectrometry experiments, including homogenization of tissue, protein concentration measurements, protei...

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Autores principales: Dowling, Paul, Gargan, Stephen, Zweyer, Margit, Henry, Michael, Meleady, Paula, Swandulla, Dieter, Ohlendieck, Kay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757555/
https://www.ncbi.nlm.nih.gov/pubmed/33377090
http://dx.doi.org/10.1016/j.xpro.2020.100196
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author Dowling, Paul
Gargan, Stephen
Zweyer, Margit
Henry, Michael
Meleady, Paula
Swandulla, Dieter
Ohlendieck, Kay
author_facet Dowling, Paul
Gargan, Stephen
Zweyer, Margit
Henry, Michael
Meleady, Paula
Swandulla, Dieter
Ohlendieck, Kay
author_sort Dowling, Paul
collection PubMed
description This protocol describes the comparative proteomic profiling of the spleen of wild type versus mdx-4cv mouse, a model of dystrophinopathy. We detail sample preparation for bottom-up proteomic mass spectrometry experiments, including homogenization of tissue, protein concentration measurements, protein digestion, and removal of interfering chemicals. We then describe the steps for mass spectrometric analysis and bioinformatic evaluation. For complete details on the use and execution of this protocol, please refer to Dowling et al. (2020).
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spelling pubmed-77575552020-12-28 Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen Dowling, Paul Gargan, Stephen Zweyer, Margit Henry, Michael Meleady, Paula Swandulla, Dieter Ohlendieck, Kay STAR Protoc Protocol This protocol describes the comparative proteomic profiling of the spleen of wild type versus mdx-4cv mouse, a model of dystrophinopathy. We detail sample preparation for bottom-up proteomic mass spectrometry experiments, including homogenization of tissue, protein concentration measurements, protein digestion, and removal of interfering chemicals. We then describe the steps for mass spectrometric analysis and bioinformatic evaluation. For complete details on the use and execution of this protocol, please refer to Dowling et al. (2020). Elsevier 2020-12-03 /pmc/articles/PMC7757555/ /pubmed/33377090 http://dx.doi.org/10.1016/j.xpro.2020.100196 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Dowling, Paul
Gargan, Stephen
Zweyer, Margit
Henry, Michael
Meleady, Paula
Swandulla, Dieter
Ohlendieck, Kay
Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen
title Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen
title_full Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen
title_fullStr Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen
title_full_unstemmed Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen
title_short Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen
title_sort protocol for the bottom-up proteomic analysis of mouse spleen
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757555/
https://www.ncbi.nlm.nih.gov/pubmed/33377090
http://dx.doi.org/10.1016/j.xpro.2020.100196
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