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Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen

This protocol describes the comparative proteomic profiling of the spleen of wild type versus mdx-4cv mouse, a model of dystrophinopathy. We detail sample preparation for bottom-up proteomic mass spectrometry experiments, including homogenization of tissue, protein concentration measurements, protei...

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Detalles Bibliográficos
Autores principales:	Dowling, Paul, Gargan, Stephen, Zweyer, Margit, Henry, Michael, Meleady, Paula, Swandulla, Dieter, Ohlendieck, Kay
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2020
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757555/ https://www.ncbi.nlm.nih.gov/pubmed/33377090 http://dx.doi.org/10.1016/j.xpro.2020.100196

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