Chronic Cranial Window for Imaging Cortical Activity in Head-Fixed Mice

Chronic cranial window surgery is a critical procedure for in vivo imaging in neuroscience. Here, we describe our surgical protocol with several subtle improvements that increase the success rate significantly. The window allows high-quality imaging in head-fixed behaving mice within the first week...

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Detalles Bibliográficos
Autores principales: Augustinaite, Sigita, Kuhn, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757562/
https://www.ncbi.nlm.nih.gov/pubmed/33377088
http://dx.doi.org/10.1016/j.xpro.2020.100194
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author Augustinaite, Sigita
Kuhn, Bernd
author_facet Augustinaite, Sigita
Kuhn, Bernd
author_sort Augustinaite, Sigita
collection PubMed
description Chronic cranial window surgery is a critical procedure for in vivo imaging in neuroscience. Here, we describe our surgical protocol with several subtle improvements that increase the success rate significantly. The window allows high-quality imaging in head-fixed behaving mice within the first week after the surgical procedure and remains clear for months. We used this procedure to prepare mice for intrinsic signal imaging and two-photon imaging of layer 6 neurons in visual cortex. For complete details on the use and execution of this protocol, please refer to Augustinaite and Kuhn (2020).
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spelling pubmed-77575622020-12-28 Chronic Cranial Window for Imaging Cortical Activity in Head-Fixed Mice Augustinaite, Sigita Kuhn, Bernd STAR Protoc Protocol Chronic cranial window surgery is a critical procedure for in vivo imaging in neuroscience. Here, we describe our surgical protocol with several subtle improvements that increase the success rate significantly. The window allows high-quality imaging in head-fixed behaving mice within the first week after the surgical procedure and remains clear for months. We used this procedure to prepare mice for intrinsic signal imaging and two-photon imaging of layer 6 neurons in visual cortex. For complete details on the use and execution of this protocol, please refer to Augustinaite and Kuhn (2020). Elsevier 2020-12-04 /pmc/articles/PMC7757562/ /pubmed/33377088 http://dx.doi.org/10.1016/j.xpro.2020.100194 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Augustinaite, Sigita
Kuhn, Bernd
Chronic Cranial Window for Imaging Cortical Activity in Head-Fixed Mice
title Chronic Cranial Window for Imaging Cortical Activity in Head-Fixed Mice
title_full Chronic Cranial Window for Imaging Cortical Activity in Head-Fixed Mice
title_fullStr Chronic Cranial Window for Imaging Cortical Activity in Head-Fixed Mice
title_full_unstemmed Chronic Cranial Window for Imaging Cortical Activity in Head-Fixed Mice
title_short Chronic Cranial Window for Imaging Cortical Activity in Head-Fixed Mice
title_sort chronic cranial window for imaging cortical activity in head-fixed mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757562/
https://www.ncbi.nlm.nih.gov/pubmed/33377088
http://dx.doi.org/10.1016/j.xpro.2020.100194
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