Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing

Tissue-clearing technologies have developed rapidly in the past decade, especially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer metastasis wi...

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Autores principales: Takahashi, Kei, Kubota, Shimpei I., Ehata, Shogo, Ueda, Hiroki R., Miyazono, Kohei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757565/
https://www.ncbi.nlm.nih.gov/pubmed/33377085
http://dx.doi.org/10.1016/j.xpro.2020.100191
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author Takahashi, Kei
Kubota, Shimpei I.
Ehata, Shogo
Ueda, Hiroki R.
Miyazono, Kohei
author_facet Takahashi, Kei
Kubota, Shimpei I.
Ehata, Shogo
Ueda, Hiroki R.
Miyazono, Kohei
author_sort Takahashi, Kei
collection PubMed
description Tissue-clearing technologies have developed rapidly in the past decade, especially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer metastasis with single-cell resolution at whole mouse body/organ level. This approach can also analyze the tumor characteristics with high-quality 3D images. Here, we describe a detailed CUBIC cancer protocol from tissue clearing, capturing 3D images and post-immunohistochemistry. For complete details on the use and execution of this protocol, please refer to Kubota et al. (2017).
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spelling pubmed-77575652020-12-28 Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing Takahashi, Kei Kubota, Shimpei I. Ehata, Shogo Ueda, Hiroki R. Miyazono, Kohei STAR Protoc Protocol Tissue-clearing technologies have developed rapidly in the past decade, especially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer metastasis with single-cell resolution at whole mouse body/organ level. This approach can also analyze the tumor characteristics with high-quality 3D images. Here, we describe a detailed CUBIC cancer protocol from tissue clearing, capturing 3D images and post-immunohistochemistry. For complete details on the use and execution of this protocol, please refer to Kubota et al. (2017). Elsevier 2020-11-26 /pmc/articles/PMC7757565/ /pubmed/33377085 http://dx.doi.org/10.1016/j.xpro.2020.100191 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Takahashi, Kei
Kubota, Shimpei I.
Ehata, Shogo
Ueda, Hiroki R.
Miyazono, Kohei
Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing
title Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing
title_full Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing
title_fullStr Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing
title_full_unstemmed Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing
title_short Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing
title_sort protocol for imaging and analysis of mouse tumor models with cubic tissue clearing
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757565/
https://www.ncbi.nlm.nih.gov/pubmed/33377085
http://dx.doi.org/10.1016/j.xpro.2020.100191
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