An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis

This protocol provides the steps required for a mouse liver orthotopic implantation model. The reliable pre-clinical animal models that have similar characteristics to hepatocellular carcinoma (HCC) are a powerful tool to unveil the mechanisms controlling tumor initiation and progression. Here, we d...

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Detalles Bibliográficos
Autores principales: Kasashima, Hiroaki, Duran, Angeles, Cid-Diaz, Tania, Kudo, Yotaro, Diaz-Meco, Maria T., Moscat, Jorge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757567/
https://www.ncbi.nlm.nih.gov/pubmed/33377079
http://dx.doi.org/10.1016/j.xpro.2020.100185
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author Kasashima, Hiroaki
Duran, Angeles
Cid-Diaz, Tania
Kudo, Yotaro
Diaz-Meco, Maria T.
Moscat, Jorge
author_facet Kasashima, Hiroaki
Duran, Angeles
Cid-Diaz, Tania
Kudo, Yotaro
Diaz-Meco, Maria T.
Moscat, Jorge
author_sort Kasashima, Hiroaki
collection PubMed
description This protocol provides the steps required for a mouse liver orthotopic implantation model. The reliable pre-clinical animal models that have similar characteristics to hepatocellular carcinoma (HCC) are a powerful tool to unveil the mechanisms controlling tumor initiation and progression. Here, we describe a syngeneic orthotopic HCC model that recapitulates the role of a host pro-tumorigenic microenvironment by pre-conditioning mouse livers with a high-fat diet (HFD). For complete details on the use and execution of this protocol, please refer to Kudo et al. (2020).
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spelling pubmed-77575672020-12-28 An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis Kasashima, Hiroaki Duran, Angeles Cid-Diaz, Tania Kudo, Yotaro Diaz-Meco, Maria T. Moscat, Jorge STAR Protoc Protocol This protocol provides the steps required for a mouse liver orthotopic implantation model. The reliable pre-clinical animal models that have similar characteristics to hepatocellular carcinoma (HCC) are a powerful tool to unveil the mechanisms controlling tumor initiation and progression. Here, we describe a syngeneic orthotopic HCC model that recapitulates the role of a host pro-tumorigenic microenvironment by pre-conditioning mouse livers with a high-fat diet (HFD). For complete details on the use and execution of this protocol, please refer to Kudo et al. (2020). Elsevier 2020-11-20 /pmc/articles/PMC7757567/ /pubmed/33377079 http://dx.doi.org/10.1016/j.xpro.2020.100185 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kasashima, Hiroaki
Duran, Angeles
Cid-Diaz, Tania
Kudo, Yotaro
Diaz-Meco, Maria T.
Moscat, Jorge
An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis
title An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis
title_full An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis
title_fullStr An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis
title_full_unstemmed An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis
title_short An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis
title_sort orthotopic implantation mouse model of hepatocellular carcinoma with underlying liver steatosis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757567/
https://www.ncbi.nlm.nih.gov/pubmed/33377079
http://dx.doi.org/10.1016/j.xpro.2020.100185
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