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Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons
This protocol enables the quantification of odor-evoked calcium activity in mushroom body Kenyon cells of the Drosophila melanogaster brain at the single bouton level. We also present subsequent characterization of naive and learned odor representations in the context of olfactory coding. This appro...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757656/ https://www.ncbi.nlm.nih.gov/pubmed/33377104 http://dx.doi.org/10.1016/j.xpro.2020.100210 |
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author | Hancock, Clare E. Geurten, Bart R.H. Fiala, André |
author_facet | Hancock, Clare E. Geurten, Bart R.H. Fiala, André |
author_sort | Hancock, Clare E. |
collection | PubMed |
description | This protocol enables the quantification of odor-evoked calcium activity in mushroom body Kenyon cells of the Drosophila melanogaster brain at the single bouton level. We also present subsequent characterization of naive and learned odor representations in the context of olfactory coding. This approach to analyzing the neuronal basis of associative learning provides a substrate for similar studies, perhaps in other animals, to probe the attributes of a neuronal memory trace at the level of synapses distributed across neurons. For complete details on the use and execution of this protocol, please refer to Bilz et al. (2020). |
format | Online Article Text |
id | pubmed-7757656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-77576562020-12-28 Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons Hancock, Clare E. Geurten, Bart R.H. Fiala, André STAR Protoc Protocol This protocol enables the quantification of odor-evoked calcium activity in mushroom body Kenyon cells of the Drosophila melanogaster brain at the single bouton level. We also present subsequent characterization of naive and learned odor representations in the context of olfactory coding. This approach to analyzing the neuronal basis of associative learning provides a substrate for similar studies, perhaps in other animals, to probe the attributes of a neuronal memory trace at the level of synapses distributed across neurons. For complete details on the use and execution of this protocol, please refer to Bilz et al. (2020). Elsevier 2020-12-15 /pmc/articles/PMC7757656/ /pubmed/33377104 http://dx.doi.org/10.1016/j.xpro.2020.100210 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Hancock, Clare E. Geurten, Bart R.H. Fiala, André Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons |
title | Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons |
title_full | Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons |
title_fullStr | Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons |
title_full_unstemmed | Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons |
title_short | Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons |
title_sort | visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single drosophila mushroom body neurons |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757656/ https://www.ncbi.nlm.nih.gov/pubmed/33377104 http://dx.doi.org/10.1016/j.xpro.2020.100210 |
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