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Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons

This protocol enables the quantification of odor-evoked calcium activity in mushroom body Kenyon cells of the Drosophila melanogaster brain at the single bouton level. We also present subsequent characterization of naive and learned odor representations in the context of olfactory coding. This appro...

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Autores principales: Hancock, Clare E., Geurten, Bart R.H., Fiala, André
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757656/
https://www.ncbi.nlm.nih.gov/pubmed/33377104
http://dx.doi.org/10.1016/j.xpro.2020.100210
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author Hancock, Clare E.
Geurten, Bart R.H.
Fiala, André
author_facet Hancock, Clare E.
Geurten, Bart R.H.
Fiala, André
author_sort Hancock, Clare E.
collection PubMed
description This protocol enables the quantification of odor-evoked calcium activity in mushroom body Kenyon cells of the Drosophila melanogaster brain at the single bouton level. We also present subsequent characterization of naive and learned odor representations in the context of olfactory coding. This approach to analyzing the neuronal basis of associative learning provides a substrate for similar studies, perhaps in other animals, to probe the attributes of a neuronal memory trace at the level of synapses distributed across neurons. For complete details on the use and execution of this protocol, please refer to Bilz et al. (2020).
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spelling pubmed-77576562020-12-28 Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons Hancock, Clare E. Geurten, Bart R.H. Fiala, André STAR Protoc Protocol This protocol enables the quantification of odor-evoked calcium activity in mushroom body Kenyon cells of the Drosophila melanogaster brain at the single bouton level. We also present subsequent characterization of naive and learned odor representations in the context of olfactory coding. This approach to analyzing the neuronal basis of associative learning provides a substrate for similar studies, perhaps in other animals, to probe the attributes of a neuronal memory trace at the level of synapses distributed across neurons. For complete details on the use and execution of this protocol, please refer to Bilz et al. (2020). Elsevier 2020-12-15 /pmc/articles/PMC7757656/ /pubmed/33377104 http://dx.doi.org/10.1016/j.xpro.2020.100210 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Hancock, Clare E.
Geurten, Bart R.H.
Fiala, André
Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons
title Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons
title_full Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons
title_fullStr Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons
title_full_unstemmed Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons
title_short Visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single Drosophila mushroom body neurons
title_sort visualization of naive and learned odor representations using in vivo calcium imaging and immunohistochemical bouton mapping of single drosophila mushroom body neurons
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757656/
https://www.ncbi.nlm.nih.gov/pubmed/33377104
http://dx.doi.org/10.1016/j.xpro.2020.100210
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