A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses

We describe the production of single-cycle (sc) and replication-competent recombinant vesicular stomatitis viruses (rcVSVs) displaying heterologous envelope glycoproteins (Envs) on their surface. We prepare scVSVs by transiently expressing HIV-1 Envs or SARS-CoV-2 spike followed by infection of the...

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Detalles Bibliográficos
Autores principales: Farzani, Touraj Aligholipour, Chov, Angela, Herschhorn, Alon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757661/
https://www.ncbi.nlm.nih.gov/pubmed/33377103
http://dx.doi.org/10.1016/j.xpro.2020.100209
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author Farzani, Touraj Aligholipour
Chov, Angela
Herschhorn, Alon
author_facet Farzani, Touraj Aligholipour
Chov, Angela
Herschhorn, Alon
author_sort Farzani, Touraj Aligholipour
collection PubMed
description We describe the production of single-cycle (sc) and replication-competent recombinant vesicular stomatitis viruses (rcVSVs) displaying heterologous envelope glycoproteins (Envs) on their surface. We prepare scVSVs by transiently expressing HIV-1 Envs or SARS-CoV-2 spike followed by infection of the cells with scVSV particles, which do not carry the vsv-g gene. To prepare rcVSVs, we replace the vsv-g with a specific env-encoding gene, transfect cells with multiple plasmids for production of the genomic RNA and viral proteins, and rescue replication-competent viruses.
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spelling pubmed-77576612020-12-28 A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses Farzani, Touraj Aligholipour Chov, Angela Herschhorn, Alon STAR Protoc Protocol We describe the production of single-cycle (sc) and replication-competent recombinant vesicular stomatitis viruses (rcVSVs) displaying heterologous envelope glycoproteins (Envs) on their surface. We prepare scVSVs by transiently expressing HIV-1 Envs or SARS-CoV-2 spike followed by infection of the cells with scVSV particles, which do not carry the vsv-g gene. To prepare rcVSVs, we replace the vsv-g with a specific env-encoding gene, transfect cells with multiple plasmids for production of the genomic RNA and viral proteins, and rescue replication-competent viruses. Elsevier 2020-12-09 /pmc/articles/PMC7757661/ /pubmed/33377103 http://dx.doi.org/10.1016/j.xpro.2020.100209 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Farzani, Touraj Aligholipour
Chov, Angela
Herschhorn, Alon
A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses
title A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses
title_full A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses
title_fullStr A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses
title_full_unstemmed A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses
title_short A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses
title_sort protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757661/
https://www.ncbi.nlm.nih.gov/pubmed/33377103
http://dx.doi.org/10.1016/j.xpro.2020.100209
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