Cargando…

Visualizing Cellular Dynamics and Protein Localization in 3D Collagen

Immune cells migrate and communicate through cell-to-cell interactions and cytokines to coordinate the specificity and timing of the immune response. While studying these events in cell culture are standard procedure, spatiotemporal dynamics of cell-to-cell interactions within three-dimensional (3D)...

Descripción completa

Detalles Bibliográficos
Autores principales:	Koh, Wan Hon, Zayats, Romaniya, Lopez, Paul, Murooka, Thomas T.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2020
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757667/ https://www.ncbi.nlm.nih.gov/pubmed/33377097 http://dx.doi.org/10.1016/j.xpro.2020.100203

Ejemplares similares

Visualizing the In Vivo Dynamics of Anti-Leishmania Immunity: Discoveries and Challenges
por: Zayats, Romaniya, et al.
Publicado: (2021)

HPV and the Risk of HIV Acquisition in Women
por: Zayats, Romaniya, et al.
Publicado: (2022)

Identifying physiological tissue niches that support the HIV reservoir in T cells
por: Ikeogu, Nnamdi, et al.
Publicado: (2023)

HIV-Captured DCs Regulate T Cell Migration and Cell-Cell Contact Dynamics to Enhance Viral Spread
por: Koh, Wan Hon, et al.
Publicado: (2020)

Histological analysis of invasive glioblastoma organoids embedded in a 3D collagen matrix
por: Guyon, Joris, et al.
Publicado: (2023)

Protocol for mitochondrial isolation and sub-cellular localization assay for mitochondrial proteins
por: Zhou, Danyi, et al.
Publicado: (2023)

Integrated functional neuronal network analysis of 3D silk-collagen scaffold-based mouse cortical culture
por: Dingle, Yu-Ting L., et al.
Publicado: (2021)

Protocol to Fabricate Engineered Illumination Devices for Optogenetic Control of Cellular Signaling Dynamics
por: Repina, Nicole A., et al.
Publicado: (2020)

A computational pipeline to visualize DNA-protein binding states using dSMF data
por: Rao, Satyanarayan, et al.
Publicado: (2022)

Quantitative 3D analysis and visualization of cardiac fibrosis by microcomputed tomography
por: Janbandhu, Vaibhao, et al.
Publicado: (2021)

Two-color in vitro assay to visualize and quantify microtubule shaft dynamics
por: Andreu-Carbó, Mireia, et al.
Publicado: (2022)

A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry
por: Pang, Zhengyuan, et al.
Publicado: (2022)

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
por: Meghezi, Sébastien, et al.
Publicado: (2015)

Real-time visualization of exo- and endocytosis membrane dynamics with confocal and super-resolution microscopy
por: Guo, Xiaoli, et al.
Publicado: (2022)

Protocol for visualizing conditional interaction between transmembrane and cytoplasmic proteins
por: Ooki, Takuya, et al.
Publicado: (2021)

Protocol for visualizing newly synthesized proteins in primary mouse hepatocytes
por: Shen, Yuqian, et al.
Publicado: (2021)

Visualizing Microbial Community Dynamics via a Controllable Soil Environment
por: Bhattacharjee, Arunima, et al.
Publicado: (2020)

Contrast-Enhanced Tissue Processing of Fibrillin-Rich Elastic Fibres for 3D Visualization by Volume Scanning Electron Microscopy
por: Lewis, Philip N., et al.
Publicado: (2021)

Imaging the Impact of Chemically Inducible Proteins on Cellular Dynamics In Vivo
por: Leong, Hon S., et al.
Publicado: (2012)

In Situ DNA/Protein Interaction Assay to Visualize Transcriptional Factor Activation
por: Corsini, Michela, et al.
Publicado: (2020)

Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions
por: Canesin, Giacomo, et al.
Publicado: (2021)

Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture
por: Semper, Cameron, et al.
Publicado: (2023)

Protocol for photoactivation of YAP in cancer cell spheroids embedded in collagen gels
por: Illes, Bernhard, et al.
Publicado: (2021)

Method of micro‐sampling human dentine collagen for stable isotope analysis
por: Curtis, Mandi J., et al.
Publicado: (2022)

Detection of thermal shift in cellular Keap1 by protein-protein interaction inhibitors using immunoblot- and fluorescence microplate-based assays
por: Dayalan Naidu, Sharadha, et al.
Publicado: (2022)

Protocol to determine the subcellular localization of protein interactions in murine keratinocytes
por: Müller, Lisa
Publicado: (2023)

Visualization and quantification of the stromal-vascular compartment in fetal or adult mouse bones: From sampling to high-resolution 3D image analysis
por: Peredo, Nicolas, et al.
Publicado: (2022)

Standardized protocol and outcome measurements for the collagen antibody-induced arthritis mouse model
por: Maleitzke, Tazio, et al.
Publicado: (2022)

A practical guide to deep-learning light-field microscopy for 3D imaging of biological dynamics
por: Zhu, Lanxin, et al.
Publicado: (2023)

Combining FRET and optical tweezers to study RhoGTPases spatio-temporal dynamics upon local stimulation
por: Iseppon, Federico, et al.
Publicado: (2017)

Synthesis of (124)I-labeled epichaperome probes and assessment in visualizing pathologic protein-protein interaction networks in tumor bearing mice
por: Sharma, Sahil, et al.
Publicado: (2022)

Analysis of local protein accumulation kinetics by live-cell imaging in yeast systems
por: Okada, Hiroki, et al.
Publicado: (2021)

Protocol for the identification and expression analysis of a cytoplasmic membrane-localized protein STING
por: Shi, Jiaqi, et al.
Publicado: (2023)

HDX-guided EPR spectroscopy to interrogate membrane protein dynamics
por: Lane, Benjamin J., et al.
Publicado: (2022)

Cellular immunotherapy for refractory hematological malignancies
por: Reagan, John L, et al.
Publicado: (2013)

Efficient RNA and RNA-protein co-detection in 3D colonoids by whole-mount staining
por: Atanga, Roger, et al.
Publicado: (2022)

Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike protein
por: Liu, Angela Rose, et al.
Publicado: (2023)

Preparation of chaperone-loaded neural stem cell-derived extracellular vesicles to reduce protein aggregation in Huntington’s disease cellular models
por: Joshi, Bhagyashree S., et al.
Publicado: (2023)

Compression loading-induced stress responses in intervertebral disc cells encapsulated in 3D collagen constructs
por: Chooi, Wai Hon, et al.
Publicado: (2016)

Visualizing carboxyl-terminal domain of RNA polymerase II recruitment by FET fusion protein condensates with DNA curtains
por: Zuo, Linyu, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_uattd53q8jbi9atv9vkeiu3alm