Quantitative photoconversion analysis of internal molecular dynamics in stress granules and other membraneless organelles in live cells

Photoconversion enables real-time labeling of protein sub-populations inside living cells, which can then be tracked with submicrometer resolution. Here, we detail the protocol of comparing protein dynamics inside membraneless organelles in live HEK293T cells using a CRISPR-Cas9 PABPC1-Dendra2 marke...

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Detalles Bibliográficos
Autores principales: Amen, Triana, Kaganovich, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757677/
https://www.ncbi.nlm.nih.gov/pubmed/33377110
http://dx.doi.org/10.1016/j.xpro.2020.100217

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757677/
https://www.ncbi.nlm.nih.gov/pubmed/33377110
http://dx.doi.org/10.1016/j.xpro.2020.100217

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