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Quantitative photoconversion analysis of internal molecular dynamics in stress granules and other membraneless organelles in live cells
Photoconversion enables real-time labeling of protein sub-populations inside living cells, which can then be tracked with submicrometer resolution. Here, we detail the protocol of comparing protein dynamics inside membraneless organelles in live HEK293T cells using a CRISPR-Cas9 PABPC1-Dendra2 marke...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757677/ https://www.ncbi.nlm.nih.gov/pubmed/33377110 http://dx.doi.org/10.1016/j.xpro.2020.100217 |