Two-parameter single-molecule analysis for measurement of chromatin mobility

This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-ta...

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Detalles Bibliográficos
Autores principales: Lerner, Jonathan, Gómez-García, Pablo Aurelio, McCarthy, Ryan L., Liu, Zhe, Lakadamyali, Melike, Zaret, Kenneth S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757679/
https://www.ncbi.nlm.nih.gov/pubmed/33377115
http://dx.doi.org/10.1016/j.xpro.2020.100223
Descripción
Sumario:This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-tagged, chromatin-binding proteins, it associates chromatin interaction properties with known functions in a way that previously used SMT parameters did not. For complete information on the use and execution of this protocol, please refer to Lerner et al. (2020).

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