Two-parameter single-molecule analysis for measurement of chromatin mobility

This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-ta...

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Detalles Bibliográficos
Autores principales: Lerner, Jonathan, Gómez-García, Pablo Aurelio, McCarthy, Ryan L., Liu, Zhe, Lakadamyali, Melike, Zaret, Kenneth S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757679/
https://www.ncbi.nlm.nih.gov/pubmed/33377115
http://dx.doi.org/10.1016/j.xpro.2020.100223
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author Lerner, Jonathan
Gómez-García, Pablo Aurelio
McCarthy, Ryan L.
Liu, Zhe
Lakadamyali, Melike
Zaret, Kenneth S.
author_facet Lerner, Jonathan
Gómez-García, Pablo Aurelio
McCarthy, Ryan L.
Liu, Zhe
Lakadamyali, Melike
Zaret, Kenneth S.
author_sort Lerner, Jonathan
collection PubMed
description This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-tagged, chromatin-binding proteins, it associates chromatin interaction properties with known functions in a way that previously used SMT parameters did not. For complete information on the use and execution of this protocol, please refer to Lerner et al. (2020).
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spelling pubmed-77576792020-12-28 Two-parameter single-molecule analysis for measurement of chromatin mobility Lerner, Jonathan Gómez-García, Pablo Aurelio McCarthy, Ryan L. Liu, Zhe Lakadamyali, Melike Zaret, Kenneth S. STAR Protoc Protocol This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-tagged, chromatin-binding proteins, it associates chromatin interaction properties with known functions in a way that previously used SMT parameters did not. For complete information on the use and execution of this protocol, please refer to Lerner et al. (2020). Elsevier 2020-12-13 /pmc/articles/PMC7757679/ /pubmed/33377115 http://dx.doi.org/10.1016/j.xpro.2020.100223 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lerner, Jonathan
Gómez-García, Pablo Aurelio
McCarthy, Ryan L.
Liu, Zhe
Lakadamyali, Melike
Zaret, Kenneth S.
Two-parameter single-molecule analysis for measurement of chromatin mobility
title Two-parameter single-molecule analysis for measurement of chromatin mobility
title_full Two-parameter single-molecule analysis for measurement of chromatin mobility
title_fullStr Two-parameter single-molecule analysis for measurement of chromatin mobility
title_full_unstemmed Two-parameter single-molecule analysis for measurement of chromatin mobility
title_short Two-parameter single-molecule analysis for measurement of chromatin mobility
title_sort two-parameter single-molecule analysis for measurement of chromatin mobility
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757679/
https://www.ncbi.nlm.nih.gov/pubmed/33377115
http://dx.doi.org/10.1016/j.xpro.2020.100223
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